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High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities
A Specificity Map for the PDZ Domain Family
The specificity map enables the prediction and prioritization of natural protein interactions, which can be used to guide PDZ domain cell biology experiments, and indicates that many viruses produce PDZ ligands that disrupt host protein complexes for their own benefit.
Ubiquitin Chain Editing Revealed by Polyubiquitin Linkage-Specific Antibodies
Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast
The development of a novel proteomic in vitro ubiquitination screen using a protein microarray platform that can be utilized for the discovery of substrates for E3 ligases on a global scale is described.
High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation.
Bayesian Modeling of the Yeast SH3 Domain Interactome Predicts Spatiotemporal Dynamics of Endocytosis Proteins
A genome-scale specificity and interaction map for yeast SH3 domain-containing proteins reveal how family members show selective binding to target proteins and predicts the dynamic localization of…
The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways
It is demonstrated that the enteropathogenic Escherichia coli (EPEC) type III effector protein EspF nucleates a multiprotein signaling complex composed of eukaryotic sorting nexin 9 (SNX9) and neuronal Wiskott-Aldrich syndrome protein (N-WASP).
Regulation of a Late Phase of T Cell Polarity and Effector Functions by Crtam
Crystal structure of full-length KcsA in its closed conformation
- S. Uysal, V. Vásquez, A. Kossiakoff
- BiologyProceedings of the National Academy of Sciences
- 21 April 2009
Functional analysis of the full-length KcsA–Fab complex suggests that the C-terminal bundle remains whole during gating, suggesting that this structure likely represents the physiologically relevant closed conformation of KCSA.