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Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli.
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A comprehensive microbial insight into two-stage anaerobic digestion of food waste-recycling wastewater.
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Methanogenic population dynamics assessed by real-time quantitative PCR in sludge granule in upflow anaerobic sludge blanket treating swine wastewater.
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Real-time PCR determination of rRNA gene copy number: absolute and relative quantification assays with Escherichia coli
TLDR
Real-time polymerase chain reaction (PCR)-based methodology for the determination of rRNA gene (rrn) copy number was introduced and demonstrated and was successfully applied to determine the rrn copy number in E. coli cells.
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Qualitative and quantitative assessment of microbial community in batch anaerobic digestion of secondary sludge.
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Primer and probe sets for group‐specific quantification of the genera Nitrosomonas and Nitrosospira using real‐time PCR
TLDR
The primer and probe sets designed in this study can be used to detect and quantify the β‐proteobacterial AOB in biological nitrification processes and various environments.
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Variations in methanogenic population structure under overloading of pre-acidified high-strength organic wastewaters
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Prevalence of antibiotic resistance genes from effluent of coastal aquaculture, South Korea.
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Monitoring bacterial and archaeal community shifts in a mesophilic anaerobic batch reactor treating a high-strength organic wastewater.
TLDR
The results demonstrated that the bacterial community shifted, reflecting the performance changes and, particularly, that a significant community shift corresponded to a considerable process event, suggesting that the diagnosis of an anaerobic digestion process could be possible by monitoring bacterial community shifts.
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Bioaugmentation of anaerobic sludge digestion with iron-reducing bacteria: process and microbial responses to variations in hydraulic retention time
TLDR
Overall, the combined biostimulation and bioaugmentation investigated in this study proved effective for enhanced methane recovery from anaerobic WAS digestion, which suggests an interesting potential for high-rate AD.
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