S. Shi

Publications51
h-index17
Citations1,347
Highly Influential Citations67
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Improving Production of Malonyl Coenzyme A-Derived Metabolites by Abolishing Snf1-Dependent Regulation of Acc1
TLDR
It was found that introduction of two site mutations in Acc1, Ser659 and Ser1157, resulted in an enhanced activity of Acc1 and increased total fatty acid content and the generation of a more efficient ACCase was applied in the production of two high-value compounds derived from malonyl-CoA, i.e., fatty acid ethyl esters that can be used as biodiesel and 3-hydroxypropionic acid.
Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production
TLDR
Five WSs from different species were functionally expressed and their substrate preference characterized in S. cerevisiae, thus constructing cell factories for the production of specific kinds of wax ester.
A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae.
Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol degradation pathway and expression of the heterologous phosphoketolase pathway
TLDR
Two different strategies for engineering of the central carbon metabolism for efficient provision of acetyl-CoA and NADPH required for fatty acid biosynthesis and hence FAEE production were evaluated and it was found that both the ethanol degradation pathway as well as the phosphoketolase pathway improve the yield of FAEEs.
Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid ethyl esters, an advanced biofuel, by eliminating non-essential fatty acid utilization pathways
Engineering of chromosomal wax ester synthase integrated Saccharomyces cerevisiae mutants for improved biosynthesis of fatty acid ethyl esters
TLDR
A chromosome engineering method based on delta integration was applied in Saccharomyces cerevisiae for the production of fatty acid ethyl esters (FAEEs), which can be directly used as biodiesel and would be a possible substitute for conventional petroleum‐based diesel.
A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
TLDR
A gRNA-tRNA array for CRISPR-Cas9 (GTR-CRISPR) for multiplexed engineering of Saccharomyces cerevisiae is reported, resulting in a 30-fold increase in free fatty acid production in 10 days using just two-round deletions of eight previously identified genes.
Transcriptome analysis guided metabolic engineering of Bacillus subtilis for riboflavin production.
Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae
TLDR
The engineered yeast strain represents a promising alternative platform for n-butanol production through a synergistic pathway: the endogenous threonine pathway and the introduced citramalate pathway.
Increased production of riboflavin by metabolic engineering of the purine pathway in Bacillus subtilis
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