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Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34.
SCFCdc4 (Skp1, Cdc53/cullin, F-box protein) defines a family of modular ubiquitin ligases (E3s) that regulate diverse processes including cell cycle, immune response, and development. MassExpand
Mobile DNA III
This new edition of the bestselling series on movable genetic elements highlights the many exciting advances in the field over the last decade, including conservative site-specific recombination,Expand
Proteolytic processing of Ty3 proteins is required for transposition.
Ty3 is a retroviruslike element found in Saccharomyces cerevisiae. It encodes GAG3 and GAG3-POL3 polyproteins which are processed into mature proteins found in the Ty3 viruslike particle. In thisExpand
Ty3 integrates within the region of RNA polymerase III transcription initiation.
Over 190 independent insertions into target plasmids of the retrovirus-like element Ty3 were recovered and mapped. Ty3 was shown to insert upstream of tRNA, 5S, and U6 genes, all of which areExpand
Ty3, a yeast retrotransposon associated with tRNA genes, has homology to animal retroviruses.
Ty3, a retrotransposon of Saccharomyces cerevisiae, is found within 20 base pairs (bp) of the 5' ends of different tRNA genes. Determination of the complete nucleotide sequence of one Ty3Expand
Retroviruses and yeast retrotransposons use overlapping sets of host genes.
A collection of 4457 Saccharomyces cerevisiae mutants deleted for nonessential genes was screened for mutants with increased or decreased mobilization of the gypsylike retroelement Ty3. Of these, 64Expand
A vector set for systematic metabolic engineering in Saccharomyces cerevisiae
A set of shuttle vectors was constructed to facilitate expression of genes for metabolic engineering in Saccharomyces cerevisiae. Selectable markers include the URA3, TRP1, MET15, LEU2‐d8, HIS3 andExpand
Small heat shock protein suppression of Vpr-induced cytoskeletal defects in budding yeast.
Expression of the auxiliary human immunodeficiency virus type 1 (HIV-1) protein Vpr causes arrest of primate host cells in G2. Expression of this protein in budding yeast has been previously reportedExpand
Host Factors That Affect Ty3 Retrotransposition in Saccharomyces cerevisiae
The retrovirus-like element Ty3 of Saccharomyces cerevisiae integrates at the transcription initiation region of RNA polymerase III. To identify host genes that affect transposition, a collection ofExpand
Membrane stress caused by octanoic acid in Saccharomyces cerevisiae
In order to compete with petroleum-based fuel and chemicals, engineering a robust biocatalyst that can convert renewable feedstocks into biorenewable chemicals, such as carboxylic acids, isExpand