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Molecular and cellular processing of lung surfactant 1
Synthesis of surfactant lipids and proteins is developmentally regulated in fetal lung and can be accelerated by glucocorticoids and other hormones and in isolated type II cells it can be stimulated by physiological and other agents that act via at least three signal‐transduction mechanisms.
Regulation of surfactant secretion.
  • S. Rooney
  • Biology, Medicine
    Comparative biochemistry and physiology. Part A…
  • 1 May 2001
There is increasing information on the identity of several of the signaling proteins involved in surfactant secretion although others remain to be established and Distal steps in the secretory pathway beyond protein kinase activation are major issues that need to be addressed.
Single plate separation of lung phospholipids including disaturated phosphatidylcholine.
An improved thin-layer chromatographic method for separation of lung phospholipids that should prove useful in studies on lung surfactant metabolism and secretion, in prediction of fetal lung maturity by analysis of amniotic fluid phospholIPids, and in Studies on surfactants obtained by bronchoscopy in adult lung diseases.
The surfactant system and lung phospholipid biochemistry.
  • S. Rooney
  • Medicine
    The American review of respiratory disease
  • 14 May 2015
Ontogeny of surfactant secretion in type II pneumocytes from fetal, newborn, and adult rats.
Differential maturation of the signal-transduction pathways mediating surfactant secretion in type II cells from developing rats is suggested, suggesting that the adenosine A1 receptor inhibiting secretion does not become functional until that age.
Purinoceptor agonists stimulate phosphatidylcholine secretion in primary cultures of adult rat type II pneumocytes.
The stimulatory effect of ATP was diminished by alpha, beta-methylene ATP but only slightly by 8-phenyltheophylline, suggesting that, although part of the ATP effect could be explained by catabolism to adenosine, the P2 purinoceptor may also be involved in regulation of surfactant secretion.
Pulmonary type II cell hypertrophy and pulmonary lipoproteinosis are features of chronic IL-13 exposure.
Interleukin (IL-13) may play a central role in the broad range of chronic pulmonary conditions in which fibrosis, type II cell hypertrophy, and surfactant accumulation occur and surfactant proteins showed two- to threefold increases, whereas SP-D increased 70-fold.
Development of glycogen and phospholipid metabolism in fetal and newborn rat lung.
No changes in phosphatidylcholine enzyme activities were noted during gestation, but both choline phosphate cytidylyltransferase and phosphatidate phosphatase activity increased after birth, and the possible contributions of carbohydrate derived from fetal lung glycogen to phospholipid synthesis are discussed.
Phosphatidylcholine synthesis and glycogen depletion in fetal mouse lung: developmental changes and the effects of dexamethasone.
The data show that the normal development profile of these parameters of fetal lung maturation in the mouse, as well as the effects of glucocorticoids thereon, are generally similar to those in the rabbit and rat, but stimulation of cholinephosphotransferase by glucoc Corticoids has not been generally observed in other species.
Isolation and Characterization of 2-Keto-3-Deoxyoctonate-Lipid A from a Heptose-Deficient Mutant of Escherichia coli
A heptose-deficient mutant of Escherichia coli has been isolated and from it a glycolipid, consisting of lipid A and 2-keto-3-deoxyoctonate (KDO), has been extracted with diisobutylketone-acetic