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A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma
TLDR
The isolation of a complementary DNA segment that detects a chromosomal segment having the properties of the gene at this locus is described, which is expressed in many tumour types, but no RNA transcript has been found in retinoblastomas and osteosarcomas.
Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR.
TLDR
A method combining immunomagnetic separation (IMS) and real-time (5'-nuclease) PCR was developed to detect Escherichia coli O157:H7 from ground beef without the need for an enrichment culture step.
P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin in neutrophil aggregation.
TLDR
It is suggested that L-selectin and PSGL-1 support a collisional cell-cell interaction that represents the first step in neutrophil aggregation.
Antibacterial action of a novel functionalized chitosan-arginine against Gram-negative bacteria.
TLDR
Studies using fluorescent probes, 1-N-phenyl-naphthylamine (NPN), nile red (NR) and propidium iodide (PI), and field emission scanning electron microscopy (FESEM) suggest that chitosan-arginine's antibacterial activity is, at least in part, due to its interaction with the cell membrane, in which it increases membrane permeability.
Basic fibroblast growth factor fused to a signal peptide transforms cells
TLDR
It is reported that bFGF, as specified by a recombinant plasmid, is itself unable to induce transformation, but acquires this ability after fusion with a secretory-signal sequence, and the resulting transformants undergo unusual morphological alteration and display tumorigenicity.
Hydroxamate-based metalloprotease inhibitor blocks shedding of L-selectin adhesion molecule from leukocytes: functional consequences for neutrophil aggregation.
TLDR
It is shown that a hydroxamate-based metalloprotease inhibitor, N-(D,L-[2-(hydroxyaminocarbonyl)- methyl]-4-methylpentano)-L-3-(tert-butyl)-alanyl-L-alanine, 2-aminoethyl amide, also inhibits L-selectin shedding from neutrophils, eosinophils, and lymphocytes.
Sulfhydryl Regulation of L-Selectin Shedding: Phenylarsine Oxide Promotes Activation-Independent L-Selectin Shedding from Leukocytes1
TLDR
Phenylarsine oxide (PAO), a trivalent arsenical that interacts with vicinal dithiols, is most potent in inducing rapid shedding of L-selectin from isolated neutrophils, eosinophils, and lymphocytes as well as from neutrophil in whole blood.
Rose Bengal-decorated silica nanoparticles as photosensitizers for inactivation of gram-positive bacteria.
A new type of photosensitizer, made from Rose Bengal (RB)-decorated silica (SiO(2)-NH(2)-RB) nanoparticles, was developed to inactivate gram-positive bacteria, including Methicillin-resistant
A highly sensitive immuno-PCR assay for detecting Group A Streptococcus.
A highly sensitive hybrid assay, based on immuno polymerase chain reaction (immuno-PCR) and enzyme-linked immunosorbent assay (ELISA) techniques, was developed for the detection of pathogenic Group A
Extracellular protein disulfide isomerase regulates ligand-binding activity of αMβ2 integrin and neutrophil recruitment during vascular inflammation.
TLDR
The results provide the first evidence that extracellular PDI could be a novel therapeutic target for preventing and treating inappropriate neutrophil sequestration and regulates αMβ2 integrin clustering.
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