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Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins
  • D. Lew, S. Reed
  • Biology, Medicine
  • The Journal of cell biology
  • 2 March 1993
In the case of pre-bud site assembly following START, it is found that the actin rearrangement could be triggered by Cln/Cdc28 activation in the absence of de novo protein synthesis, suggesting that the kinase may directly phosphorylate substrates (such as actin-binding proteins) that regulate actin distribution in cells. Expand
Acceleration of the G1/S phase transition by expression of cyclins D1 and E with an inducible system.
Results suggest that cyclins D1 and E are rate-limiting activators of the G1-to-S phase transition and that cyclin D1 might play a specialized role in facilitating emergence from quiescence. Expand
Physical interaction of the retinoblastoma protein with human D cyclins
It is concluded that cyclins D1 and D3 interact with pRb in a fashion distinct from cyclins A and E, which can induce pRB hyperphosphorylation, and that cyclin D1 activity may be regulated by its association with p Rb. Expand
Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell line
It is shown that turnover of phosphorylated cyclin E depends on an SCF-type protein-ubiquitin ligase that contains the human homologue of yeast Cdc4, which is an F-box protein containing repeated sequences of WD40. Expand
Regulation of retinoblastoma protein functions by ectopic expression of human cyclins
It is shown that constitutively expressed cyclins A and E can overcome pRB-mediated suppression of proliferation and suggest that G1 and S phase cyclins can act as regulators of pRb function in the cell cycle by promoting pR b phosphorylation. Expand
Association of human cyclin E with a periodic G1-S phase protein kinase.
The cyclin E-Cdk2 complex may constitute a human G1-S phase-specific regulatory protein kinase, which controls the G1 to S phase transition in budding yeast. Expand
A family of cyclin homologs that control the G1 phase in yeast.
Two Saccharomyces cerevisiae genes were isolated based upon their dosage-dependent rescue of a temperature-sensitive mutation of the gene CDC28, which encodes a protein kinase involved in control ofExpand
A CDK-independent function of mammalian Cks1: targeting of SCF(Skp2) to the CDK inhibitor p27Kip1.
It is demonstrated that Cks1 directs the ubiquitin-mediated proteolysis of the CDK-bound substrate p27Kip1 by the protein ubiquit in ligase (E3) SCF(Skp2), which is essential for recognition of the p27kip1 substrate for ubiquitination in vivo and in vitro. Expand
Stn1, a new Saccharomyces cerevisiae protein, is implicated in telomere size regulation in association with Cdc13.
It is proposed that Stn1 functions in telomere metabolism during late S phase in cooperation with Cdc13, an essential Saccharomyces cerevisiae gene, as a suppressor of the cdc13-1 mutation. Expand
p53-dependent inhibition of cyclin-dependent kinase activities in human fibroblasts during radiation-induced G1 arrest
The data suggest a model in which ionizing radiation confers G1 arrest via the p53-mediated induction of a Cdk inhibitor protein, and it is found that both whole-cell lysates and inactive cyclin E-Cdk2 complexes prepared from irradiated cells contained an activity capable of inactivating cyclinE-C DK2 complexes. Expand