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Biochemical properties and substrate specificities of alkaline and histidine acid phytases
This review describes recent findings that present novel viewpoints concerning the molecular basis of phytase classification and exhibits strict substrate specificity for the calcium–phytate complex and produces myo-inositol trisphosphate as the final product. Expand
Gene cloning and characterization of thermostable lipase from Bacillus stearothermophilus L1.
The gene coding for an extracellular lipase of Bacillus stearothermophilus L1 was cloned in Escherichia coli and showed highest activity toward p-nitrophenyl caprylate among the synthetic substrates and tripropionin among the triglycerides. Expand
An efficient direct induction of protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture
High-frequency protocorm-like body (PLB) formation directly from thin leaf sections of Doritaenopsis hybrid was achieved in order to develop a mass-scale propagation system and light microscopy observations indicated that the initial cell divisions for PLB formation occurred on the region near the cut surface and that an intact epidermal layer appeared to play an important role inPLB formation. Expand
A Lipid Transfer–like Protein Is Necessary for Lily Pollen Tube Adhesion to an in Vitro Stylar Matrix
Immunolocalization data support the role of the 9-kD protein in facilitating adhesion of pollen tubes to the stylar transmitting tract epidermis in species such as lily. Expand
A Lily Stylar Pectin Is Necessary for Pollen Tube Adhesion to an in Vitro Stylar Matrix
In vitro binding assays revealed that this lily stylar adhesive pectin and SCA are able to bind to each other in a pH-dependent manner. Expand
Hyperhydricity in apple: ultrastuctural and physiological aspects
The activities of antioxidant enzymes, such as superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase were higher in hyperhydric leaves than in healthy leaves, indicating thathyperhydricity was associated with oxidative stress. Expand
New cold-adapted lipase from Photobacterium lipolyticum sp. nov. that is closely related to filamentous fungal lipases
Results indicate that the M37 lipase from P. lipolyticum sp. Expand
Purification and characterization of a novel chitinase from the entomopathogenic fungus, Metarhizium anisopliae.
A novel chitinase was detected in extracellular culture fluids of the entomopathogenic fungus Metarhizium anisopliae (ATCC 20500) grown in liquid medium containing chitin as a sole carbon source. AExpand
Protocorm-like body induction and subsequent plant regeneration from root tip cultures of Doritaenopsis
Among the cytokinins tested, TDZ was found to be more effective cytokinin in the induction of PLBs than benzyladenine (BA) or zeatin, and callus-mediated PLB induction from cortical cells was found. Expand
Characterization of glk, a gene coding for glucose kinase of Corynebacterium glutamicum.
It was found that glk was expressed constitutively under all tested conditions with different carbon sources and the growth defect of glk mutant on rich medium was complemented by a plasmid-encoded glk gene. Expand