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A simplified method of quantitating protein using the biuret and phenol reagents.
Abstract A new modification of the Lowry method of quantitating protein is introduced, whereby the protein sample is mixed first with a diluted biuret reagent and later with 2 n phenol reagentExpand
Kinetic studies of calcium release from sarcoplasmic reticulum in vitro.
It is suggested that kinetically identical channels are responsible for Ca2+ release independent of the methods of triggering and this in vitro release is consistent with the physiological mechanism both in terms of the rapidity and the reversibility of Ca2+, as determined by stopped flow spectrometry of changes in [CaO2+]. Expand
Nitric oxide production and perivascular nitration in brain after carbon monoxide poisoning in the rat.
It is concluded that perivascular reactions mediated by peroxynitrite are important in the cascade of events which lead to brain oxidative stress in CO poisoning. Expand
A Possible Site of Superoxide Generation in the Complex I Segment of Rat Heart Mitochondria
The results suggest that the major site of superoxide generation is not flavin, but protein-associated ubisemiquinones which are spin-coupled with iron-sulfer cluster N2. Expand
Characterization of the murexide method: dual-wavelength spectrophotometry of cations under physiological conditions.
  • S. Ohnishi
  • Chemistry, Medicine
  • Analytical biochemistry
  • 1 March 1978
It is concluded that the unique features of the murexide method are (i) low binding constant of indicators for Ca 2+ and (ii) insensitivity to Mg 2+ in an aqueous solution. Expand
Stimulation of perivascular nitric oxide synthesis by oxygen.
Findings highlight the complexity of oxidative stress responses and may help explain some of the dose responses associated with therapeutic applications of hyperbaric oxygen. Expand
Central Nervous System Trauma: Research Techniques
The Mouse Head Injury Model: Utility in the Discovery of Acute Cerebroprotective Agents and the Assessment of Cognitive Function Following Traumatic Brain Injury, C.H. Mullen, Jr. Expand
Sickle cell anemia: a potential nutritional approach for a molecular disease.
Both an ex vivo study and a pilot clinical trial demonstrated that a cocktail consisting of daily doses of 6 g of aged garlic extract, 4-6 g of vitamin C, and 800 to 1200 IU of vitamin E may indeed be beneficial to the patients. Expand
Relationship between free radical production and lipid peroxidation during ischemia-reperfusion injury in the rat brain
The results suggest that the lipid peroxidation may be the direct consequence of the action of free radicals formed during ischemia and reperfusion periods. Expand
Kinetic studies of Ca2+ release from sarcoplasmic reticulum of normal and malignant hyperthermia susceptible pig muscles.
The time-course of Ca2+ release from sarcoplasmic reticulum isolated from muscles of normal pigs and those of pigs susceptible to malignant hyperthermia were investigated using stopped-flowExpand