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Dynamic pathways of -1 translational frameshifting
TLDR
This work applies single-molecule fluorescence to track the compositional and conformational dynamics of individual ribosomes at each codon during translation of a frameshift-inducing mRNA from the dnaX gene in Escherichia coli, highlighting multiple kinetic branchpoints during elongation.
Coordinated conformational and compositional dynamics drive ribosome translocation
TLDR
This work uses single-molecule fluorescence with zero-mode waveguides to directly correlate ribosome conformation and composition during multiple rounds of elongation at high factor concentrations in Escherichia coli.
O-phospho-L-serine and the thiocarboxylated sulfur carrier protein CysO-COSH are substrates for CysM, a cysteine synthase from Mycobacterium tuberculosis.
TLDR
This study represents the first detailed kinetic characterization of sulfide transfer from a sulfide carrier protein and interpret this finding to suggest that the CysM active site with the bound aminoacrylate intermediate is protected from solvent and that binding of CysO-COSH produces a conformational change allowing rapid sulfur transfer.
N6-methyladenosine in mRNA disrupts tRNA selection and translation elongation dynamics
TLDR
Bulk kinetic and single-molecule methods and measurements in an Escherichia coli translation system revealed that m6A modification of mRNA acts as a barrier to tRNA accommodation and translation elongation, demonstrating that chemical modification of RNA can change translational dynamics.
Glycal formation in crystals of uridine phosphorylase.
TLDR
NMR time course studies demonstrate that uridine phosphorylase can catalyze the hydrolysis of the fluorinated nucleosides in the absence of phosphate or sulfate, without the release of intermediates or enzyme inactivation.
Transcription–translation coupling: direct interactions of RNA polymerase with ribosomes and ribosomal subunits
TLDR
It is reported that RNA polymerase directly binds ribosomes and isolated large and small ribosomal subunits, forming a one-to-one complex with a micromolar dissociation constant.
Analysis of the carbapenem gene cluster of Erwinia carotovora: definition of the antibiotic biosynthetic genes and evidence for a novel β‐lactam resistance mechanism
TLDR
The genetic dissection of this putative operon is reported to determine the function of each of the genes, and it is demonstrated by mutational analysis that the products of the first five genes of the operon are involved in the synthesis of the carbapenem molecule.
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