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Complete genome sequence and comparative analysis of the industrial microorganism Streptomyces avermitilis
The complete nucleotide sequence of the linear chromosome of Streptomyces avermitilis is determined and it is revealed that an internal 6.5-Mb region in the S. avermitILis genome was highly conserved with respect to gene order and content, and contained all known essential genes but showed perfectly asymmetric structure at the oriC center. Expand
Degradation of CFTR by the ubiquitin-proteasome pathway
It is shown that the degradation of both wild-type and mutant CFTR is inhibited by two potent proteasome inhibitors that induce the accumulation of polyubiquitinated forms of immature CFTR, confirming that ubiquitination is required for rapid CFTR degradation. Expand
Genome sequence of an industrial microorganism Streptomyces avermitilis: Deducing the ability of producing secondary metabolites
Streptomyces avermitilis is a soil bacterium that carries out not only a complex morphological differentiation but also the production of secondary metabolites, one of which, avermectin, isExpand
Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism
The suitability of the mutants as hosts for efficient production of foreign metabolites was shown by heterologous expression of three different exogenous biosynthetic gene clusters encoding the biosynthesis of streptomycin, cephamycin C, and pladienolide. Expand
Avermectins, New Family of Potent Anthelmintic Agents: Producing Organism and Fermentation
The avermectins are a complex of chemically related agents which exhibit extraordinarily potent anthelmintic activity. They are produced by a novel species of actinomycete, NRRL 8165, which we haveExpand
The Degradation of Apolipoprotein B100 Is Mediated by the Ubiquitin-proteasome Pathway and Involves Heat Shock Protein 70*
It is shown that increasing the expression of Hsp70 in HepG2 cells promotes apoB degradation and is the first example of a wild-type mammalian protein whose secretion is regulated by degradation in the cytosol via the ubiquitin-proteasome pathway. Expand
The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis.
  • S. O̅mura
  • Chemistry, Medicine
  • Bacteriological reviews
  • 1 September 1976
The present review deals with studies, which have hitherto been reported, on the production, isolation, structure, and mode of action of cerulenin and its application as a biochemical tool. Expand
Structural and Computational Analysis of the Quinone-binding Site of Complex II (Succinate-Ubiquinone Oxidoreductase)
A mechanism for the reduction of ubiquinone during the catalytic turnover of the enzyme SQR is proposed, and two binding positions are proposed for the E. coli SQR quinone-binding site to explain these data. Expand
Atpenins, potent and specific inhibitors of mitochondrial complex II (succinate-ubiquinone oxidoreductase)
Atpenins potently and specifically inhibit the succinate-ubiquinone reductase activity of mitochondrial complex II, suggesting that atpenins may be useful tools for clarifying the biochemical and structural properties ofcomplex II, as well as for determining its physiological roles in mammalian tissues. Expand
Terpene synthases are widely distributed in bacteria
A powerful search method based on the use of hidden Markov models (HMMs) and protein families database (Pfam) search that has allowed the discovery of monoterpene synthases of bacterial origin is described. Expand