Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice.
- O. Minowa, T. Arai, T. Noda
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 11 April 2000
Exposure of DNA to endogenous oxidative species continuously produces the mutagenic adduct 8-OH-G in mice, and Mmh plays an essential role in repair of this DNA damage.
RNA signals for translation frameshift: influence of stem size and slippery sequence.
The results of in vitro translation studies indicate that the stem-loop is not essential even if its size influences the efficiency of ribosomal frameshifting, and that the optimum repetition number of a single nucleotide within the slippery sequence exists for efficient ribosome frameshifted.
TAK1 represses transcription of the human telomerase reverse transcriptase gene
It is demonstrated that TGF-β-activated kinase 1 (TAK1) represses the hTERT core promoter activity in an E-box-independent manner, and it alsoRepresses the transcription of the h TERT gene in human lung adenocarcinoma cell line, A549 cells.
Human MMH (OGG1) type 1a protein is a major enzyme for repair of 8-hydroxyguanine lesions in human cells.
- Y. Monden, T. Arai, M. Asano, E. Ohtsuka, H. Aburatani, S. Nishimura
- BiologyBiochemical and Biophysical Research…
- 19 May 1999
It is shown that upon depletion of hMMH type 1a protein in a whole cell extract by its antibody, most of the AP lyase activity is lost, indicating that h MMH type 2a protein is a major enzyme for repair of 8-OH-G lesions in human cells.
J-104,871, a novel farnesyltransferase inhibitor, blocks Ras farnesylation in vivo in a farnesyl pyrophosphate-competitive manner.
In vitro studies strongly suggest that J-series compounds have an FPP-competitive nature, and suggest that FTase inhibition in whole cells as well as in the in vitro system is in line with that of squalene synthase and FTase.
Distinct PKC isozymes regulate bufalin-induced differentiation and apoptosis in human monocytic cells.
- M. Kurosawa, Y. Tani, S. Nishimura, S. Numazawa, T. Yoshida
- Biology, ChemistryAmerican Journal of Physiology - Cell Physiology
- 1 March 2001
The findings suggest that bufalin-induced cell differentiation and apoptosis are interlinked and that distinct PKC isozymes are involved in the fate of the cell.
The hydration of Ras p21 in solution during GTP hydrolysis based on solution X-ray scattering profile.
- T. Fujisawa, T. Uruga, Z. Yamaizumi, Ȳ. Inoko, S. Nishimura, T. Ueki
- ChemistryJournal of Biochemistry (Tokyo)
- 1 May 1994
It was concluded that the ras p21 molecule incorporates 20% more bulk water upon GTP binding, and the increase of bulk water is especially conspicuous around the interface between switch I and switch II regions, which suggests that hydration plays an important role in the interaction with GAP.
Cloning of the RhoB gene from the mouse genome and characterization of its promoter region.
- T. Nakamura, M. Asano, N. Shindo-Okada, S. Nishimura, Y. Monden
- Biology, MedicineBiochemical and Biophysical Research…
- 24 September 1996
Cloned the rhoB gene from the mouse genome and characterized its promoter region, finding that the minimum region of the promoter was located between positions -507 and -376 relative to the site for initiation of translation.
Regulation of ERK-mediated signal transduction by p38 MAP kinase in human monocytic THP-1 cells.
- S. Numazawa, M. Watabe, S. Nishimura, M. Kurosawa, Makoto Izuno, Takemi Yoshida
- BiologyJournal of Biochemistry (Tokyo)
- 1 May 2003
It is demonstrated that SB 203580 activates members of the ERK cascade, c-Raf, MEK, and ERK, in human monocytic THP-1 cells, suggesting that the steady-state level of p38 MAP kinase activity modulates ERK signaling.
Suppression of translation frameshift by upstream termination codon.
It is described that an upstream termination codon suppresses the ribosomal frameshifting, leading to reduction in the expression of downstream genes.