• Publications
  • Influence
Stability-increasing mutants of glucose dehydrogenase from Bacillus megaterium IWG3.
The results of this study indicate that the tetrameric structure of glucose dehydrogenase is stabilized by several kinds of mutation, and at least one of the following amino acid substitutions stabilizes the enzyme. Expand
Mechanism of 4-Nitrophenol Oxidation in Rhodococcus sp. Strain PN1: Characterization of the Two-Component 4-Nitrophenol Hydroxylase and Regulation of Its Expression
Functional analysis, in addition to the sequence analysis, revealed that the 4-NP hydroxylase system belongs to the two-component flavin-diffusible monooxygenase family. Expand
Biodegradation of nylon oligomers
  • S. Negoro
  • Biology, Medicine
  • Applied Microbiology and Biotechnology
  • 1 October 2000
A molecular basis for the adaptation of microorganisms toward xenobiotics compounds was described and two strains which initially had no metabolic activity for nylon oligomers, Flavobacterium sp. Expand
Sequence and properties of β-xylosidase from Bacillus pumilus IPO
The nucleotide sequence of the β-xylosidase (xynB) gene from Bacillus pamilus and Escherichia coli harboring a hybrid plasmid which highly expresses the xynB gene is indentified. Expand
High Production of Thermostable β-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis
By cloning the beta-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times and is suitable for application in industrial processes. Expand
Purification and characterization of a new glucose dehydrogenase from vegetative cells of Bacillus megaterium
Abstract A new type of glucose dehydrogenase was purified from vegetative cells of Bacillus megaterium IAM1030. The characteristics of the vegetative-cell enzyme were investigated and compared withExpand
Characterization of alkylphenol degradation gene cluster in Pseudomonas putida MT4 and evidence of oxidation of alkylphenols and alkylcatechols with medium-length alkyl chain.
Results suggest that Pseudomonas putida MT4 is a very useful strain in the biodegradation of a wide range of APs with a medium-length alkyl chain, which known nonylphenol-degrading Sphingomonas strains have never degraded. Expand
Identification and characterization of another 4-nitrophenol degradation gene cluster, nps, in Rhodococcus sp. strain PN1.
Results indicate that this nps gene clusters, in addition to the nph gene cluster, is also involved in 4-NP degradation in strain PN1. Expand
6-Aminohexanoate Oligomer Hydrolases from the Alkalophilic Bacteria Agromyces sp. Strain KY5R and Kocuria sp. Strain KY2
ABSTRACT Alkalophilic, nylon oligomer-degrading strains, Agromyces sp. and Kocuria sp., were isolated from the wastewater of a nylon-6 factory and from activated sludge from a sewage disposal plant.Expand
High homology between 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium and Pseudomonas strains
The nucleotide sequences of the genes for 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium sp. strain K172 (F-nylA) and Pseudomonas sp. NK87 (P-nylA), enzymes essential for the degradationExpand