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Biodegradation of nylon oligomers
- S. Negoro
- Biology, EngineeringApplied Microbiology and Biotechnology
- 1 October 2000
A molecular basis for the adaptation of microorganisms toward xenobiotics compounds was described and two strains which initially had no metabolic activity for nylon oligomers, Flavobacterium sp.
Stability-increasing mutants of glucose dehydrogenase from Bacillus megaterium IWG3.
Mechanism of 4-Nitrophenol Oxidation in Rhodococcus sp. Strain PN1: Characterization of the Two-Component 4-Nitrophenol Hydroxylase and Regulation of Its Expression
Functional analysis, in addition to the sequence analysis, revealed that the 4-NP hydroxylase system belongs to the two-component flavin-diffusible monooxygenase family.
Sequence and properties of β-xylosidase from Bacillus pumilus IPO
The nucleotide sequence of the β-xylosidase (xynB) gene from Bacillus pamilus and Escherichia coli harboring a hybrid plasmid which highly expresses the xynB gene is indentified.
Characterization of alkylphenol degradation gene cluster in Pseudomonas putida MT4 and evidence of oxidation of alkylphenols and alkylcatechols with medium-length alkyl chain.
Results suggest that Pseudomonas putida MT4 is a very useful strain in the biodegradation of a wide range of APs with a medium-length alkyl chain, which known nonylphenol-degrading Sphingomonas strains have never degraded.
Evolutionary adaptation of plasmid-encoded enzymes for degrading nylon oligomers
Comparisons of the nucleotide sequences and gene products of the nylA and nylB′ genes conclude that EII enzyme is newly evolved by gene duplication followed by base substitutions on the same plasmid.
High Production of Thermostable β-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis
- Haruhisa Hirata, S. Negoro, H. Okada
- Medicine, BiologyApplied and environmental microbiology
- 1 June 1985
By cloning the beta-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times and is suitable for application in industrial processes.
Purification and characterization of a new glucose dehydrogenase from vegetative cells of Bacillus megaterium
Solubility of artificial proteins with random sequences