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Some enzymatic properties of 3beta-hydroxysteroid oxidase produced by Streptomyces violascens.
The 3beta-hydroxysteroid oxidase produced by Streptomyces violascens was purified from the culture broth by procedures including batch-wise treatment on DEAE-cellulose, ammonium sulfate fraction, gelExpand
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Dissociation constants of glucan phosphorylases of rabbit tissues studied by polyacrylamide gel disc electrophoresis.
Using polyacrylamide gel disc electrophoresis, a simple and sensitive stain method for glucan phosphorylase (EC was developed. With this method 0.3–1.5 μg or 1–5 units of phosphorylase couldExpand
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Structure of the trypsin-binding domain of Bowman-Birk type protease inhibitor and its interaction with trypsin.
The crystal structure of the complex formed by bovine trypsin and Bowman-Birk type protease inhibitor AB-I extracted from azuki beans (Vigna angularis) 'Takara' has been analyzed. The structure wasExpand
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Properties of Aspergillus niger catalase.
Catalase from Aspergillus niger was purified to homogeneity as judged from the results of ultracentrifugation and polyacrylamide gel electrophoresis. The enzyme had a molecular weight of 385,000 asExpand
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Comparison of phosphorylation sites in protamines between protein kinase C and cAMP-dependent protein kinase.
Phosphorylation sites of protamines by protein kinase C and cAMP-dependent protein kinase (protein kinase A) were studied. Using clupeine Y1 as a substrate, protein kinase C phosphorylates both SerExpand
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Purification, amino acid sequence, and some properties of rabbit kidney lysozyme.
The lysozyme (rabbit kidney lysozyme) from the homogenate of rabbit kidney (Japanese white) was purified by repeated cation-exchange chromatography on Bio-Rex 70. The amino acid sequence wasExpand
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Studies on the ferredoxin-ferredoxin-NADP reductase complex: kinetic and solvent perturbation studies on the location of sulfhydryl and aromatic amino acid residues.
The molecular weight of spinach ferredoxin-NADP reductase [EC] was estimated to be 33,100 by the sedimentation equilibrium method. On the basis of this molecular weight, the amino acidExpand
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Action mechanism of glucose oxidase of Aspergillus niger.
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