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Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.
TLDR
The use of MSP is demonstrated to identify promoter region hypermethylation changes associated with transcriptional inactivation in four important tumor suppressor genes (p16, p15, E-cadherin and von Hippel-Lindau) in human cancer. Expand
Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer
TLDR
Although DNA methylation and histone deacetylation appear to act as synergistic layers for the silencing of genes in cancer, dense CpG island methylation is dominant for the stable maintenance of a silent state at these loci. Expand
Hypermethylation of the APC (adenomatous Polyposis Coli) gene promoter region in human colorectal carcinoma
TLDR
It is shown that the promoter region of the APC gene is heavily methylated at CpG sites in patients with colorectal carcinoma in comparison with normal colonic mucosa and premalignant adenomas, suggesting that cytosine methylation of the regulatory sequences of theAPC gene could be involved in the progression of human coloreCTal cancer. Expand
Mapping Patterns of CpG Island Methylation in Normal and Neoplastic Cells Implicates Both Upstream and Downstream Regions inde Novo Methylation*
TLDR
Data suggest that boundaries exist at both ends of a CpG island to maintain the unmethylated state in normal tissue and that these boundaries may be progressively overridden, eliciting the de novo methylation associated with tumor suppressor gene silencing in neoplasia. Expand
Tissue-specific expression of the diazepam-binding inhibitor in Drosophila melanogaster: cloning, structure, and localization of the gene.
TLDR
It is proposed that in D. melanogaster, DBI is involved in energy metabolism in a manner that depends on the substrate used for energy production, on the basis of the expression of DBI in some but not all tissues with high energy consumption. Expand
Hypermethylation can selectively silence individual p16ink4A alleles in neoplasia.
TLDR
In neoplastic cells, stable allele-specific loss of transcription may arise from aberrant methylation of a nonmutated promoter region, identifying hypermethylation as a direct mechanism for tumor suppressor gene inactivation. Expand
Sequence-specific DNA Binding Activity of RNA Helicase A to thep16INK4a Promoter*
TLDR
RNA helicase A (RHA) is identified as a protein that binds much better to thep16INK4a promoter in the expressing cells and is shown to be a cellular substrate for caspase-3, which decreases its sequence-specific binding top16ink4a by cleavage of the N terminus. Expand
High-level expression of bovine β-lactoglobulin gene in transgenic mice
TLDR
Bovine BLG gene was isolated from a genomic library and introduced into murine germline and genomic sequencing indicated that the promoter region was heavily methylated except for distinct CpG sites that were only partially methylated both in transgenic mice and lactating cattle. Expand
Generation of Transgenic Dairy Cattle from Transgene-Analyzed and Sexed Embryos Produced In Vitro
TLDR
A transgenic calf was generated from in vitro produced bovine embryos which had undergone transgene analysis and sexing prior to the embryo transfer and was found to be transgenic by PCR analysis from blood, ear and fetal membranes. Expand
Transgenic mice aberrantly expressing human ornithine decarboxylase gene.
TLDR
The offspring of the first transgenic male founder animal did not show any overt abnormalities, yet their reproductive performance was reduced, and the second transgenic founder animal, showing similar aberrant expression of ornithine decarboxylase in all tissues studied, was found to be infertile. Expand
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