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Cryo-electron microscopy of the vacuolar ATPase motor reveals its mechanical and regulatory complexity.
The vacuolar H+-ATPase (V-ATPase) is an ATP-driven rotary molecular motor that is a transmembrane proton pump in all eukaryotic cells. Although its activity is fundamental to many physiological… Expand
Triclosan inhibits the growth of Plasmodium falciparum and Toxoplasma gondii by inhibition of apicomplexan Fab I.
- R. McLeod, S. Muench, +10 authors D. Rice
- Materials Science, Biology
- International journal for parasitology
- 1 February 2001
Fab I, enoyl acyl carrier protein reductase (ENR), is an enzyme used in fatty acid synthesis. It is a single chain polypeptide in plants, bacteria, and mycobacteria, but is part of a complex… Expand
Cryo-EM structures of complex I from mouse heart mitochondria in two biochemically defined states
Complex I (NADH:ubiquinone oxidoreductase) uses the reducing potential of NADH to drive protons across the energy-transducing inner membrane and power oxidative phosphorylation in mammalian… Expand
The essential GTPase YphC displays a major domain rearrangement associated with nucleotide binding
- S. Muench, Ling Xu, S. Sedelnikova, D. Rice
- Biology, Medicine
- Proceedings of the National Academy of Sciences
- 15 August 2006
The structure of a Bacillus subtilis YphC/GDP complex shows that it contains two GTPase domains that pack against a central domain whose fold resembles that of an RNA binding KH-domain. Comparisons… Expand
Structural divergence of the rotary ATPases.
The rotary ATPase family of membrane protein complexes may have only three members, but each one plays a fundamental role in biological energy conversion. The F₁F(o)-ATPase (F-ATPase) couples ATP… Expand
Maternal Inheritance and Stage-Specific Variation of the Apicoplast in Toxoplasma gondii during Development in the Intermediate and Definitive Host
ABSTRACT The structure and location of Toxoplasma gondii apicoplasts were examined in intermediate and definitive hosts and shown to vary in a stage-specific manner. Immunocytochemistry and electron… Expand
A method for detergent-free isolation of membrane proteins in their local lipid environment
Despite the great importance of membrane proteins, structural and functional studies of these proteins present major challenges. A significant hurdle is the extraction of the functional protein from… Expand
An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology
- Rebecca F. Thompson, M. Walker, C. A. Siebert, S. Muench, N. Ranson
- Medicine, Biology
- 1 May 2016
Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins >150 kDa. There are several… Expand
Replace, reuse, recycle: improving the sustainable use of phosphorus by plants.
- A. Baker, S. A. Ceasar, +4 authors S. Baldwin
- Medicine, Biology
- Journal of experimental botany
- 1 June 2015
The 'phosphorus problem' has recently received strong interest with two distinct strands of importance. The first is that too much phosphorus (P) is entering into waste water, creating a significant… Expand
Methods to account for movement and flexibility in cryo-EM data processing
Recent advances in direct electron detectors and improved CMOS cameras have been accompanied by the development of a range of software to take advantage of the data they produce. In particular they… Expand