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Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
This chapter describes techniques concerned with classical and molecular genetics, cell biology, and biochemistry that can be used with Schizosaccharomyces pombe. Expand
Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
It is found that genes of similar functions are clustered in distinct, multi-megabase regions of individual chromosomes; genes in these regions tend to share transcriptional profiles. Expand
The genome sequence of Schizosaccharomyces pombe
The genome of fission yeast (Schizosaccharomyces pombe), which contains the smallest number of protein-coding genes yet recorded for a eukaryote, is sequenced and highly conserved genes important for eukARYotic cell organization including those required for the cytoskeleton, compartmentation, cell-cycle control, proteolysis, protein phosphorylation and RNA splicing are identified. Expand
Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1
It is concluded that both Cds1 and Chk1 regulate the binding of Cdc25 to 14-3-3 proteins as part of the checkpoint response to unreplicated DNA. Expand
Genomic stability and tumour suppression by the APC/C cofactor Cdh1
Results indicate that Cdh1 is required for preventing unscheduled proliferation of specific progenitor cells and protecting mammalian cells from genomic instability. Expand
Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis.
A fission yeast orthologue of CDC14 (cdc fourteen-like-phosphatase) null mutant is identified, viable, divides at a reduced size and shows defects in septation, and the potential roles of flp1p in cell cycle control are discussed. Expand
Regulation of p34cdc2 protein kinase during mitosis
The cdc25+, cdc13+, and suc1+ gene products are important for regulating p34cdc2 protein kinase activity during entry into, progress through, and exit from mitosis. Expand
Regulation of the G1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor
In fission yeast, the cyclin‐dependent kinase (CDK) inhibitor p25rum1 is a key regulator of progression through the G1 phase of the cell cycle. We show here that p25rum1 protein levels are sharplyExpand
Fission yeast Tor2 promotes cell growth and represses cell differentiation
This work demonstrates that Tor2, a fission yeast member of the TOR protein kinase family, is central to controlling the switch between cell growth and cell differentiation in response to nutrient availability and reveals a unique regulatory function of the Tor pathway – ensuring that growth andcell differentiation become mutually exclusive and that the choice between them depends on environmental conditions. Expand