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A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor in Arabidopsis
TLDR
It is reported that the basic leucine zipper transcription factor ABI5 confers an enhanced response to exogenous ABA during germination, and seedling establishment, as well as subsequent vegetative growth.
ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination.
TLDR
Results indicate that ABI5 acts downstream of ABI3 to reactivate late embryogenesis programmes and to arrest growth of germinating embryos.
Lipid Rafts in Higher Plant Cells
TLDR
The data clearly indicate that tobacco microdomains are able to recruit a specific set of the plasma membrane proteins and exclude others, and it is demonstrated the recruitment of the NADPH oxidase after elicitation by cryptogein and the presence of the small G protein NtRac5, a negative regulator of NADPH oxidation, in lipid rafts.
AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation.
TLDR
It is shown that a novel ABI5-interacting protein, designated as AFP, can form high molecular weight (Mr) complexes with A BI5 in embryo-derived extracts, and suggests that AFP attenuates ABA signals by targeting ABA5 for ubiquitin-mediated degradation in nuclear bodies.
A MYB Transcription Factor Regulates Very-Long-Chain Fatty Acid Biosynthesis for Activation of the Hypersensitive Cell Death Response in Arabidopsis[W][OA]
TLDR
Microarray analyses of Arabidopsis plants misexpressing MYB30 show that the genes encoding the four enzymes forming the acyl-coA elongase complex are putative MyB30 targets, and a model in which MYB 30 modulates HR via VLCFAs by themselves, or VLCFA derivatives, as cell death messengers in plants is proposed.
Characterization of Lipid Rafts from Medicago truncatula Root Plasma Membranes: A Proteomic Study Reveals the Presence of a Raft-Associated Redox System1[W]
TLDR
It is shown that lipid raft domains, defined as Triton X-100-insoluble membranes, can also be prepared from Medicago truncatula root PMs, and that receptor kinases and proteins related to signaling, cellular trafficking, and cell wall functioning were well represented whereas those involved in transport and metabolism were poorly represented.
Proteomics of Plant Detergent-resistant Membranes * S
TLDR
Analysis of the lipidic composition of a tobacco plasma membrane fraction resistant to solubilization with Triton X-100 revealed its enrichment in sphingolipids and sterols and depletion in phospho- and glycerolipidas as previously observed for animal microdomains.
Remorin, a Solanaceae Protein Resident in Membrane Rafts and Plasmodesmata, Impairs Potato virus X Movement[W]
TLDR
It is shown that REM can interact physically with the movement protein TRIPLE GENE BLOCK PROTEIN1 from PVX and the potential for lipid rafts to act as functional components in plasmodesmata and the plasma membrane is discussed.
A remorin protein interacts with symbiotic receptors and regulates bacterial infection
TLDR
It is shown that this oligomeric remorin protein attaches to the host plasma membrane surrounding the bacteria and controls infection and release of rhizobia into the host cytoplasm.
Cell wall constrains lateral diffusion of plant plasma-membrane proteins
TLDR
Fluorescence recovery after photobleaching is used to estimate mobility of a set of minimal PM proteins and it is confirmed that the pattern of cellulose deposition in the cell wall affects the trajectory and speed of PM protein diffusion.
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