S. Miyairi

Publications31
h-index7
Citations242
Highly Influential Citations0
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The preparation of steroid N-hydroxysuccinimide esters and their reactivities with bovine serum albumin.
The N-hydroxysuccinimide esters of testosterone and cortisol were prepared from the corresponding carboxylic acid derivatives by a carbodiimide method. These activated esters were covalently linked… Expand
  • 61
Sensitivity and specificity in enzyme immunoassay of testosterone.
The effects of the combination of antiserum and enzyme-labeled steroid and of the molar ratio of steroid to enzyme in the preparation of the conjugate on sensitivity and specificity in enzyme… Expand
  • 33
The specificity in enzyme immunoassay for plasma and urine cortisol.
  • 7
Radioimmunoassay of 16α-hydroxyestrone in human urine
Abstract A radioimmunoassay for the quantitation of the sum of free, glucuronidated and urine is described. The method is reliable and accurate. Using this method, urinary excretion of… Expand
  • 13
Enantioselective immunoaffinity extraction for simultaneous determination of optically active bufuralol and its metabolites in human plasma by HPLC.
A combined method of immunoaffinity extraction with high-performance liquid chromatography has been developed for the enantioselective determination of bufuralol and its metabolites in human plasma.… Expand
  • 17
Specificity of antisera raised against cortisol-4-bovine serum albumin conjugates in radioimmunoassay.
In order to obtain specific antisera for use in radioimmunoassay and enzyme immunoassay of cortisol, new hapten-carrier conjugates were prepared from 4-hemisuccinoyloxycortisol, 4-(carboxymethylthio)… Expand
  • 9
Determination of metallothionein by high-performance liquid chromatography with fluorescence detection using an isocratic solvent system.
Metallothionein (MT) is a low-molecular-weight protein which plays a role in detoxification of heavy metals and protection against oxidative stress. A sensitive and convenient determination method… Expand
  • 23
Novel immunoaffinity extraction for liquid chromatographic determination of major metabolites of 4-acetoxy-2-(4-methylphenyl)benzothiazole in plasma.
Group extraction of the metabolites of 4-acetoxy-2-(4-methylphenyl)benzothiazole has been achieved through the use of an immunoaffinity adsorbent. The antisera elicited from an immunogen,… Expand
  • 8
Radioimmunoassay of 11-deoxycortisol. Specificity of antisera raised against 11-deoxycortisol-[C-4]-bovine serum albumin conjugates.
In order to obtain specific antisera for use in immunoassays of 11-deoxycortisol, new hapten-carrier conjugates were prepared from 4-hemisuccinoyloxy-11-deoxycortisol,… Expand
  • 7
Immunoaffinity extraction of 4-hydroxy-2-(4-methylphenyl)benzothiazole and its metabolites for determination by gas chromatography-mass spectrometry.
Immunoaffinity extraction of 4-hydroxy-2-(4-methylphenyl)benzothiazole and its metabolites, together with the corresponding meta-isomers has been achieved by the use of an antibody raised against an… Expand
  • 3