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Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata
Changes in the cellular content of three polyamines (putrescine, spermidine and spermine) were compared at different stages of development in zygotic and somatic embryos of Pinus radiata D. Don.Expand
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Polyamines and cellular metabolism in plants: transgenic approaches reveal different responses to diamine putrescine versus higher polyamines spermidine and spermine
Distribution of biogenic amines—the diamine putrescine (Put), triamine spermidine (Spd), and tetraamine spermine (Spm)—differs between species with Put and Spd being particularly abundant and Spm theExpand
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Transgenic manipulation of the metabolism of polyamines in poplar cells.
The metabolism of polyamines (putrescine, spermidine, and spermine) has become the target of genetic manipulation because of their significance in plant development and possibly stress tolerance. WeExpand
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Polyamines and abiotic stress in plants: a complex relationship1
The physiological relationship between abiotic stress in plants and polyamines was reported more than 40 years ago. Ever since there has been a debate as to whether increased polyamines protectExpand
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Genetic Manipulation of the Metabolism of Polyamines in Poplar Cells. The Regulation of Putrescine Catabolism1
We investigated the catabolism of putrescine (Put) in a non-transgenic (NT) and a transgenic cell line of poplar (Populus nigra × maximowiczii) expressing a mouse (Mus musculus) ornithine (Orn)Expand
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Ornithine: the overlooked molecule in the regulation of polyamine metabolism.
We overexpressed a mouse ornithine decarboxylase gene under the control of a constitutive and an estradiol-inducible promoter in Arabidopsis thaliana to increase our understanding of the regulationExpand
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Chronic N-amended soils exhibit an altered bacterial community structure in Harvard Forest, MA, USA.
At the Harvard Forest, Petersham, MA, the impact of 20 years of annual ammonium nitrate application to the mixed hardwood stand on soil bacterial communities was studied using 16S rRNA genesExpand
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Increased Putrescine Biosynthesis through Transfer of Mouse Ornithine Decarboxylase cDNA in Carrot Promotes Somatic Embryogenesis
Carrot (Daucus carota L.) cells were transformed with Agrobacterium tumefaciens strains containing 3[prime]-truncated mouse ornithine decarboxylase (ODC) cDNA under the control of a cauliflowerExpand
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A rapid and reliable procedure for extraction of cellular polyamines and inorganic ions from plant tissues
A fast and reliable method for the extraction of cellular polyamines and major inorganic ions (Ca, Mg, Mn, K, and P) from several plant tissues is described. The method involves repeated freezing andExpand
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Polyamines and their biosynthetic enzymes during somatic embryo development in red spruce (Picea rubens Sarg.)
SummaryThe major objective of this study was to determine if the observed changes in polyamines and their biosynthetic enzymes during somatic embryo development were specifically related to eitherExpand
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