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All-atom empirical potential for molecular modeling and dynamics studies of proteins.
- Alexander D. MacKerell, D. Bashford, M. Karplus
- ChemistryThe journal of physical chemistry. B
- 14 April 1998
The results demonstrate that use of ab initio structural and energetic data by themselves are not sufficient to obtain an adequate backbone representation for peptides and proteins in solution and in crystals.
Atypical Membrane Topology and Heteromeric Function of Drosophila Odorant Receptors In Vivo
It is demonstrated that OR83b heterodimerizes with conventional ORs early in the endomembrane system in OSNs, couples these complexes to the conserved ciliary trafficking pathway, and is essential to maintain the OR/OR83b complex within the sensory cilia, where odor signal transduction occurs.
An in Vivo Map of the Yeast Protein Interactome
A genome-wide in vivo screen for protein-protein interactions in Saccharomyces cerevisiae by means of a protein-fragment complementation assay (PCA) identified 2770 interactions among 1124 endogenously expressed proteins, revealing a previously unexplored subspace of the yeast protein interactome.
Chromatin- and Transcription-Related Factors Repress Transcription from within Coding Regions throughout the Saccharomyces cerevisiae Genome
The results show that a large number of factors that control chromatin structure and transcription are required to repress cryptic transcription from at least 1,000 locations across the S. cerevisiae genome, and two results suggest that some cryptic transcripts are translated.
A highly sensitive protein-protein interaction assay based on Gaussia luciferase
A new PCA assay is described, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGFβ signaling.
Erythropoietin receptor activation by a ligand-induced conformation change.
An in vivo protein fragment complementation assay was used to obtain evidence for an alternative mechanism in which unliganded erythropoietin receptor dimers exist in a conformation that prevents activation of JAK2 but then undergo a ligand-induced conformation change that allows JAK1 to be activated.
Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments.
- J. Pelletier, F. Campbell-Valois, S. Michnick
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 13 October 1998
An oligomerization-assisted enzyme reassembly strategy whereby fragments are covalently linked to independently folding and interacting domains whose interactions serve to promote efficient refolding and complementation of fragments, forming active enzyme.
Weak functional constraints on phosphoproteomes.
The scaffold protein Ste5 directly controls a switch-like mating decision in yeast
It is argued that the architecture of the Fus3–Ste5–Ptc1 circuit generates a novel ultrasensitivity mechanism, which is robust to variations in the concentrations of these proteins, which helps assure that mating can occur despite stochastic or genetic variation between individuals.
PKB/Akt modulates TGF-beta signalling through a direct interaction with Smad3.
The results suggest a very simple mechanism for the integration of signals arising from growth-factor- and TGF-beta-mediated pathways, mediated by a physical interaction between the serine-threonine kinase PKB (protein kinase B)/Akt and the transcriptional activator Smad3.