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Prospective of biodiesel production utilizing microalgae as the cell factories: A comprehensive discussion

This article represents a comprehensive discussion about the potential of microalgae for the production of valuable lipid compounds that can be further used for biodiesel production.
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ISSR and RAPD Based Evaluation of Genetic Stability of Encapsulated Micro Shoots of Glycyrrhiza glabra Following 6 Months of Storage

The genetic fidelity of plants obtained after conversion of alginate beads was ascertained through 10 RAPD and 13 ISSR primers and cluster analysis from ISSR and RAPD showed that all the tested plants including the mother plant distributed in two major groups.
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ISSR and RAPD based evaluation of genetic fidelity and active ingredient analysis of regenerated plants of Picrorhiza kurroa

The similarity coefficient by both RAPD and ISSR analysis revealed that differences between tissue culture raised plants and mother plant was not remarkable, but notable differences were observed among three adventitious shoots regenerated from three calli.
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Plant regeneration, genetic fidelity, and active ingredient content of encapsulated hairy roots of Picrorhiza kurrooa Royle ex Benth.

Phytochemical analysis showed that picrotin and picrotoxinin content were similar in hairy root line and its regenerants, and transformed plants were of similar morphotype having increased leaf number and branched root system as compared to non-transformed plants.
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Efficiency of liquid culture systems over conventional micropropagation: A progress towards commercialization

Micropropagation in bioreactors for optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of plants in liquid culture systems.
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In vitro propagation of Rauwolfia serpentina using liquid medium, assessment of genetic fidelity of micropropagated plants, and simultaneous quantitation of reserpine, ajmaline, and ajmalicine.

The present optimized protocol offers an impeccable end to end method from the establishment of aseptic cultures to in-vitro plantlet production employing semisolid as well liquid nutrient culture medium and assessment of their genetic fidelity using polymerase chain reaction based rapid amplification of polymorphic DNA analysis.
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Elicitor-Induced Cellular and Molecular Events Are Responsible for Productivity Enhancement in Hairy Root Cultures: An Insight Study

It is concluded that combining elicitation with in-silico approaches enhances the usefulness of this practice in better understanding and identifying the rate-limiting steps of biosynthetic pathways existing in HRs which in turn can contribute towards better productivity by utilizing metabolic engineering aspects.
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Genetic transformation studies and scale up of hairy root culture of Glycyrrhiza glabra in bioreactor

It was found that though WP was not responsive for hairy root induction, yet all four basal media supported hairy root growth and a gradual increase in fresh weight biomass was observed with an increase in culture duration, however amongst all, the NB medium composition supported best growth of hairy roots followed by MS, B 5 and WP media.
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Molecular and chemical characterization of plants regenerated from Ri-mediated hairy root cultures of Rauwolfia serpentina

Analysis of reserpine content revealed that roots of regenerated plants had similar levels (0.0889 % DW) to those of their corresponding hairy roots, and all plants derived from hairy roots carried the Ri TL-DNA fragment.
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Hairy root biotechnology—indicative timeline to understand missing links and future outlook

This review presents an indicative timeline portrayal of hairy root research reflected by a chronology of research outputs and reveals a progressive trend in the state-of-art global advances in hairy root biotechnology.
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