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Escherichia coli Strains Engineered for Homofermentative Production of d-Lactic Acid from Glycerol
TLDR
The engineered homolactic route for d-lactate production was engineered by overexpressing pathways involved in the conversion of glycerol to this product and blocking those leading to the synthesis of competing by-products, thus representing a viable metabolic pathway.
Efficient synthesis of L-lactic acid from glycerol by metabolically engineered Escherichia coli
TLDR
This study demonstrates the efficient conversion of glycerol to L-lactate, a microbial process that had not been reported in the literature prior to this work.
Butyrate production in engineered Escherichia coli with synthetic scaffolds.
TLDR
Synthetic scaffold protein that spatially co-localizes enzymes was introduced to improve the efficiency of the heterologous pathway enzymes, resulting in threefold improvement in butyrate production.
Identification of cultivation condition to produce correctly folded form of a malaria vaccine based on Plasmodium falciparum merozoite surface protein-1 in Escherichia coli
TLDR
It was observed that the higher expression level of PfMSP-119 was essentially associated with the generation of higher level of incorrectly folded PfM SP-119, suggesting that it was highly pure, homogeneous, and correctly folded.
Spectroscopic and mechanistic studies of type-1 and type-2 copper sites in Pseudomonas aeruginosa azurin as obtained by addition of external ligands to mutant His46Gly.
TLDR
It is concluded that the hole created by the mutation, even though it is completely buried inside the protein, can be filled by external ligands, often resulting in the creation of a mixture of so-called type-1 and type-2 copper sites.
Improvement of 2,3-Butanediol Yield in Klebsiella pneumoniae by Deletion of the Pyruvate Formate-Lyase Gene
TLDR
From an analysis of the transcriptome, it was confirmed that the removal of pflB from K. pneumoniae significantly repressed the expression of genes involved in the formate hydrogen lyase (FHL) system.
The introduction of a negative charge into the hydrophobic patch of Pseudomonas aeruginosa azurin affects the electron self-exchange rate and the electrochemistry.
TLDR
It is demonstrated once again that the Cu-ligand His117 in the hydrophobic patch is the likely entry and exit point for electrons in the solution as well as for the reaction at an electrode.
Plasmodium falciparum Merozoite Surface Protein 1 (MSP-1)-MSP-3 Chimeric Protein: Immunogenicity Determined with Human-Compatible Adjuvants and Induction of Protective Immune Response
TLDR
Recombinant MSP-Fu24 was highly immunogenic in mice and in rabbits when formulated with two different human-compatible adjuvants and induced an immune response against both PfM SP-119 and PfMSP-311 and may form a part of a multicomponent malaria vaccine.
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