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Attuning hydroxypropyl methylcellulose phthalate to oral delivery vehicle for effective and selective delivery of protein vaccine in ileum.
This study represents the first demonstration of HPMCP for ileum-specific delivery of protein vaccine through oral route and induced strong antibody mediated immune responses but also generated memory T cells in the spleen as adaptive immunity indicating a direct evidence of an effective delivery system. Expand
Exploring Codon Optimization and Response Surface Methodology to Express Biologically Active Transmembrane RANKL in E. coli
This is the first time to report heterologous expression of mRANKL in soluble form and to perform a comparative study of functional properties of both forms of RANKL. Expand
Major degradable polycations as carriers for DNA and siRNA.
The recent developments on these three major degradable polycations as promising carriers for deoxyribonucleic acid (DNA) and small interfering RNA (siRNA) are explained. Expand
Tuning the buffering capacity of polyethylenimine with glycerol molecules for efficient gene delivery: staying in or out of the endosomes.
Results indicated that the buffering capacity of the polymers primarily impacts endosomal escape and subsequent transfection efficiency, and highlights the significance of cross-linkers in optimizing thebuffering capacity when designing polymers for gene delivery. Expand
Systemic administration of RANKL overcomes the bottleneck of oral vaccine delivery through microfold cells in ileum.
To the best of the knowledge, this is the first study to demonstrate significant induction of mucosal and humoral immune responses to M cell targeted oral vaccines after the systemic administration of RANKL. Expand
Microfluidics-Enabled Multimaterial Maskless Stereolithographic Bioprinting.
The proposed system provides a robust platform for bioprinting of high-fidelity multimaterial microstructures on demand for applications in tissue engineering, regenerative medicine, and biosensing, which are otherwise not readily achievable at high speed with conventional stereolithographic biofabrication platforms. Expand
Heterologous expression of metK1-sp and afsR-sp in Streptomyces venezuelae for the production of pikromycin
Two regulator genes, metK1-sp and afsR-sp, from Streptomyces peucetius ATCC 27952 were heterologously expressed in S. venezuelae to produce 14-membered pikromycin antibiotics to stimulate the expression of the pathway-specific regulatory genes, pikD and ketosynthase, as demonstrated by RT-PCR. Expand
Chitosan-based particulate systems for the delivery of mucosal vaccines against infectious diseases.
This review highlights and discusses advances in the development of chitosan-based particulate systems, specifically for the delivery of mucosal vaccines against infections. Expand
Identification and functional characterization of an afsR homolog regulatory gene from Streptomyces venezuelae ATCC 15439.
Homology-based analysis showed that afsR-sv represents a transcriptional activator belonging to the Streptomyces antibiotic regulatory protein (SARP) family that includes an Nterminal SARP domain containing a bacterial transcriptional activation domain (BTAD), an NB-ARC domain, and a Cterminal tetratricopeptide repeat domain. Expand
Nasal immunization with mannan-decorated mucoadhesive HPMCP microspheres containing ApxIIA toxin induces protective immunity against challenge infection with Actinobacillus pleuropneumoiae in mice.
The development of subunit mucosal vaccines requires an appropriate delivery system or an immune modulator such as an adjuvant to improve antigen immunogenicity. The nasal route for vaccine deliveryExpand