S. M. Thoroed
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Cell Swelling Activates Phospholipase A2 in Ehrlich Ascites Tumor Cells
- S. M. Thoroed, L. Lauritzen, I. Lambert, H. S. Hansen, E. Hoffmann
- Chemistry, Medicine
- The Journal of Membrane Biology
- 1 November 1997
Abstract. Ehrlich ascites tumor cells, loaded with 3H-labeled arachidonic acid and 14C-labeled stearic acid for two hours, were washed and transferred to either isotonic or hypotonic media containing… Expand
The Na(+)-independent taurine influx in flounder erythrocytes and its association with the volume regulatory taurine efflux.
95% of the Na(+)-independent influx of taurine in flounder erythrocytes at normal osmolality (330 mosmol kg-1) and 0.30 mmol l-1 taurine was mediated by a saturable system (Vmax = 0.689 nmol g-1 dry… Expand
Protein phosphotyrosine phosphatase inhibitors suppress regulatory volume decrease and the volume-sensitive Cl– conductance in mouse fibroblasts
Abstract The effects of the protein tyrosine phosphatase (PTP) inhibitors, pervanadate, monoperoxo(picolinato)- oxo-vanadate(V) [mpV(pic)] and dephostatin, on regulatory volume decrease (RVD) and the… Expand
Hypotonic cell swelling induces translocation of the alpha isoform of cytosolic phospholipase A2 but not the gamma isoform in Ehrlich ascites tumor cells.
We demonstrate that two isoforms of the cytosolic phospholipase A2, cPLA2alpha and cPLA2gamma, are present in Ehrlich ascites tumor cells. Both enzymes are almost uniformly distributed throughout the… Expand
Hypotonic cell swelling induces translocation of the α isoform of cytosolic phospholipase A2 but not the γ isoform in Ehrlich ascites tumor cells
We demonstrate that two isoforms of the cytosolic phospholipase A2, cPLA2a and cPLA2g, are present in Ehrlich ascites tumor cells. Both enzymes are almost uniformly distributed throughout the cells… Expand
Dual role of ATP in supporting volume-regulated chloride channels in mouse fibroblasts.
- A. Bryan-Sisneros, V. Sabanov, S. M. Thoroed, P. Doroshenko
- Medicine, Chemistry
- Biochimica et biophysica acta
- 29 September 2000
The effects of inhibitors of protein tyrosine kinases (PTKs) on the Cl(-) current (I(Cl(vol))) through volume-regulated anion/chloride (VRAC) channels whilst manipulating cellular ATP have been… Expand
Taurine transport associated with cell volume regulation in flounder erythrocytes under anisosmotic conditions.
The taurine transport of flounder erythrocytes is associated with a cell volume regulation in anisosmotic media. An osmolality reduction leads to a cell volume increase, which is followed by a volume… Expand
Taurine and volume regulation in fish cells.
Characterization of the Na+-dependent taurine influx in flounder erythrocytes
About 92% of the taurine influx in flounder erythrocytes at physiological conditions in vitro (330 mosmol·l-1, 145 mmol·l-1 Na+, 0.30 mmol·l-1 taurine) is Na+-dependent. This influx is highly… Expand
Free amino compounds and cell volume regulation in erythrocytes from different marine fish species under hypoosmotic conditions: the role of a taurine channel
Erythrocytes from the marine fish species ballan wrasse (Labrus berggylta Ascanius), bullhead (Myoxocephalus scorpius L.), cod (Gadus morhua L.), dab (Limanda limanda L.), eelpout (Zoarces viviparus… Expand