Results of dot-blot hybridization showed that one sample from Beijing and one from Hainan were positive for CbVd-3, the first report from China and the only report of this viroid outside Germany, and provide experimental evidence that the two tested plants were infected by CbvD-3.
To the authors' knowledge, this is the first report of CChMVd in chrysanthemum in China and it is thought to be the symptomatic type based on their UUUC sequence at positions 82 to 85 in the CChmVd tetraloop.
Leaves were collected from 50 symptomless coleus plants from a commercial nursery in Hainan Province, China and results suggested the presence of CbVd-2, which is a member of the Coleviroid genus, Pospoviroidae family.
To the authors' knowledge, this is the first report of FMV in fig trees in China, and this virus poses a great threat to the economy of the fig industry of Xinjiang and needs immediate effective quarantine and management of this virus to reduce any further predictable loss.
It is demonstrated that CbVd-5 could induce albino symptom on the leaves of the unknown cultivar "Red with dark green edge" 2 months after inoculation, and considering the effect of Cbvd- 5 on the appearance of coleus and its recombination ability, a certification program may be needed to control the spread of this viroid.
Sequence alignment and RNA structure analysis showed that, compared with isolates and variants reported in Italy, there were two nucleotide variations and an alteration of the secondary structure in the ADFVd variants the authors isolated.