• Publications
  • Influence
A synthetic oscillatory network of transcriptional regulators
TLDR
This work used three transcriptional repressor systems that are not part of any natural biological clock to build an oscillating network, termed the repressilator, in Escherichia coli, which periodically induces the synthesis of green fluorescent protein as a readout of its state in individual cells.
Bacterial Persistence as a Phenotypic Switch
TLDR
Investigating the persistence of single cells of Escherichia coli with the use of microfluidic devices found phenotypic switching occurred between normally growing cells and persister cells having reduced growth rates, leading to a simple mathematical description of the persistence switch.
From molecular to modular cell biology
TLDR
General principles that govern the structure and behaviour of modules may be discovered with help from synthetic sciences such as engineering and computer science, from stronger interactions between experiment and theory in cell biology, and from an appreciation of evolutionary constraints.
Phenotypic Diversity, Population Growth, and Information in Fluctuating Environments
TLDR
It is shown that stochastic switching can be favored over sensing when the environment changes infrequently, and the optimal switching rates then mimic the statistics of environmental changes.
Robustness in simple biochemical networks
TLDR
It is argued that the key properties of biochemical networks should be robust in order to ensure their proper functioning, and it is shown that this applies in particular to bacterial chemotaxis.
Protein Mobility in the Cytoplasm ofEscherichia coli
TLDR
Noninvasive measurements of the apparent diffusion coefficient of green fluorescent protein (GFP) in the cytoplasm of Escherichia coli have implications for the understanding of intracellular biochemical networks.
Establishment of developmental precision and proportions in the early Drosophila embryo
TLDR
It is shown that the Bcd gradient displays a high embryo-to-embryo variability, but that this noise in the positional information is strongly decreased (‘filtered’) at the level of hunchback (hb) gene expression.
Robustness in bacterial chemotaxis
TLDR
This work focuses on how response and adaptation to attractant signals vary with systematic changes in the intracellular concentration of the components of the chemotaxis network, and finds that some properties show strong variations in response to varying protein concentrations.
An ultrasensitive bacterial motor revealed by monitoring signaling proteins in single cells.
TLDR
M Motors from different bacteria exhibited an identical steep input-output relation, suggesting that they actively contribute to signal amplification in chemotaxis, and can be extended to quantitative in vivo studies of other biochemical networks.
Mechanisms of noise-resistance in genetic oscillators
TLDR
It is shown that this type of oscillator is driven mainly by two elements: the concentration of a repressor protein and the dynamics of an activator protein forming an inactive complex with the repressor, which makes it especially resistant to fluctuations.
...
1
2
3
4
5
...