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Effect of impact stress on microbial recovery on an agar surface.
Microbial stress due to the impaction of microorganisms onto an agar collection surface was studied experimentally. The relative recovery rates of aerosolized Pseudomonas fluorescens and MicrococcusExpand
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Asymbiotic and symbiotic seed germination of Eulophia alta (Orchidaceae)—preliminary evidence for the symbiotic culture advantage
Eulophia alta (Linnaeus) Fawcett & Rendle seeds collected from the Florida Panther National Wildlife Refuge (Collier County, FL; FPNWR) were used in a screen of five asymbiotic orchid seedExpand
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Asymbiotic seed germination and in vitro seedling development of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid
Continuing loss of native orchid habitat has lead to an increased emphasis on orchid conservation. Major obstacles in the production of native orchid seedlings for use in conservation have been: (1)Expand
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Asymbiotic seed germination, in vitro seedling development, and greenhouse acclimatization of the threatened terrestrial orchid Bletia purpurea
Procedures for asymbiotic seed germination and seedling acclimatization were developed for Bletia purpurea, a threatened North America native terrestrial orchid. Six asymbiotic orchid seedExpand
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Symbiotic germination of three semi-aquatic rein orchids (Habenaria repens, H. quinquiseta, H. macroceratitis) from Florida
The destruction of wetlands in populated areas (e.g. Florida) has prompted interest in habitat restoration. We describe a symbiotic technique to germinate seeds of three semi-aquatic rein orchidExpand
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Conservation-driven Propagation of an Epiphytic Orchid (Epidendrum nocturnum) with a Mycorrhizal Fungus
Seeds of an endangered epiphytic orchid from Florida (Epidendrum nocturnum Jacquin) germinated in vitro with a mycorrhizal fungus (Epulorhiza repens (Bernard) Moore) using a technique normallyExpand
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Symbiotic seed germination of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid
The rapid loss of native orchid habitat throughout ecologically important areas (e.g., Florida) has prompted researchers to develop appropriate plans for the propagation and reintroduction of manyExpand
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Techniques and Applications of In Vitro Orchid Seed Germination
In nature orchid seeds germinate only following infection by mycorrhizal fungi that provide the developing embryo with water, carbohydrates, minerals, and vitamins. Orchid seeds were first germinatedExpand
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Protocorm mycobionts of the Federally threatened eastern prairie fringed orchid, Platanthera leucophaea (Nutt.) Lindley, and a technique to prompt leaf elongation in seedlings
A yet unresolved question in orchid biology is whether mycorrhizal fungi (= mycobionts) utilized as a carbon source by young seedlings (= protocorms) are different from those utilized by adultExpand
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