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IAA-induced apogamy in Platycerium coronarium (Koenig) Desv. gametophytes cultured in vitro
Using light microscope and confocal laser scanning microscope, it is shown that nuclear size can be used as a quick parameter to estimate the ploidy level of P. coronarium. Expand
Morphogenetic plasticity of callus reinitiated from cell suspension cultures of the fern Platycerium coronarium
The dark-green gametophytic callus showed a faster rate of growth and morphogenesis as compared to the pale-green sporophyticCallus, and the availability of cell cultures of the same ploidy level, but with two distinct pathways of development will be useful for comparative studies of developmental plasticity. Expand
Production of first and second generations aposporous gametophytes fromPyrrosia piloselloides (L.) Price frond strips cultured in vitro
Modified medium in 1% agar supplemented with sugar alcohols, auxins, and cytokinin promoted a higher percentage of aposporous development from mature fronds of Pyrrosia piloselloides derived from aseptically cultured spores as compared with those obtained from plants in the field. Expand
Ribulose-1,5-bisphosphate carboxylase and phosphoenolpyruvate carboxylase activities of photoautotrophic callus of Platycerium coronarium (Koenig ex O.F. Muell.) Desv. under CO2 enrichment
The in vitro activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) were measured in cell-free extracts of Platycerium coronarium callusExpand
Production of sporophytes from Platycerium coronarium and P. ridleyi Frond Strips and Rhizome Pieces Cultured In Vitro
Production of P. coronarium and P. ridleyi sporophytes from stem and frond explants is reported and it is reported that the species is extremely rare. Expand
Role of ethylene in the production of sporophytes from Platycerium coronarium (Koenig) desv. frond and rhizome pieces cultured in Vitro
The biosynthetic pathway of ethylene in the fern P. coronarium appears to be different from that of higher plants but similar to that of some other ferns. Expand
Establishment and physiological analyses of photoautotrophic callus cultures of the fern Platycerium coronarium (Koenig) Desv. under CO2 enrichmen
The data are in agreement with the view that long-term exposure to high levels of CO2 can cause a decrease in photosynthetic capacity. Expand
Production of aposporous gametophytes and calli from Pteris vittata L. pinnae strips cultured in vitro
The first report of simultaneous production of calli and gametophytes from fern explants is reported, and it is reported that the calli regenerated sporophytes when cultured on MS medium without growth regulators and the gamettes grew vegetatively on MSmedium but produced sporophyte when transferred into 0.1 strength MS medium. Expand
Production of aposporous gametophytes from Drymoglossum piloselloides (L.) Presl. frond strips
Strips of Drymoglossum piloselloides fronds in a modified Murashige and Skoog medium in 1% agar produced aposporous gametophytes in about 2 months. Young fronds showed a greater ability to developExpand
Ammonium and nitrate uptake and nitrate reductase activity of photoautotrophic callus cultures of the fernPlatycerium coronarium (Koenig) DESV
The uptake of nitrate and ammonium by callus of Platycerium coronarium from the culture medium was examined and the presence of 1% (vol/vol) CO2 generally resulted in the highest rate of Nitrate uptake. Expand