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Characterization of N- and O-linked glycosylation of recombinant human bile salt-stimulated lipase secreted by Pichia pastoris.
Recombinant human bile salt-stimulated lipase (hBSSL) was expressed in and secreted by Pichia pastoris, an organism exploited for the large-scale production of recombinant (glyco)proteins byExpand
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Estrogen regulation of creatine kinase-B in the rat uterus.
Creatine kinase-B (CKB) synthesis is rapidly and specifically induced by estrogen in the uterus of the immature rat. This study indicates that this elevation is due at least in part to increases inExpand
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Estradiol preferentially enhances extracellular tissue plasminogen activators of MCF-7 breast cancer cells.
MCF-7 human breast cancer cells secrete two immunologic types of plasminogen activator, one related to urokinase, the other unrelated. We have now examined whether estrogen stimulation of secretedExpand
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Suppression of estrogen-regulated extracellular tissue plasminogen activator activity of MCF-7 cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin.
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) suppresses the estrogen enhancement of tissue plasminogen activator (t-PA) by MCF-7 breast cancer cells. 17 beta-estradiol treatment of MCF-7 cells wasExpand
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Transcriptional regulation of surfactant proteins SP-A and SP-B by phorbol ester.
Phorbol esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA) activate protein kinase C and have been previously shown to down-regulate surfactant proteins SP-A and SP-B in H441 adenocarcinomaExpand
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Estrogen regulation of human breast cancer cell line MCF-7 tissue plasminogen activator.
The human breast cancer cell line MCF-7 produces a number of estrogen-regulated proteins, among which is tissue plasminogen activator (tPA). Increased medium concentrations of PA activity wereExpand
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Inhibition of Azotobacter vinelandii RNA polymerase by cibacron blue F3GA.
  • S. Kumar, J. Krakow
  • Medicine, Chemistry
  • The Journal of biological chemistry
  • 25 August 1977
Cibacron blue F3GA is a potent inhibitor of the Azotobacter vinelandii DNA-directed RNA polymerase. Addition of 8 micrometer Cibacron blue F3GA prior to initiation results in a greater than 90%Expand
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Effect of Cibacron blue F3GA on oligonucleotide binding site of estradiol--receptor complexes of mouse uterine cytosol.
The binding of estradiol--receptor complexes of mouse uterine cytosol to oligodeoxynucleotide celluloses is inhibited by the sulfonated polyaromatic dye Cibacron blue F3GA. The dye does not have anyExpand
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Effect of chaotropic anions on the rate of dissociation of estradiol-receptor protein complexes of mouse uterine cytosol.
Abstract The stability of mouse uterine cytosol receptor-[ 3 H]estradiol complex was evaluated in the presence of neutral salts of the Hoffmeister series. Marked increases in the rate of dissociationExpand
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