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Long and accurate PCR with a mixture of KOD DNA polymerase and its exonuclease deficient mutant enzyme.
DNA polymerase from Thermococcus kodakaraensis KOD1 (previously Pyrococcus sp. KOD1) is one of the most efficient thermostable PCR enzymes exhibiting higher accuracy and elongation velocity than anyExpand
TMC-95A, B, C, and D, novel proteasome inhibitors produced by Apiospora montagnei Sacc. TC 1093. Taxonomy, production, isolation, and biological activities.
TMC-95A did not inhibit m-calpain, cathepsin L, and trypsin at 30 microM, suggesting its high selectivity for proteasome, and taxonomy of the producing strain is also described. Expand
Selective Isolation and Distribution of Actinomadura rugatobispora Strains in Soil
These results indicated that A. rugatobispora strains were distributed worldwide and were more resistant to 100 μg/ml gentamicin and 20 μg/ML ampicillin than Streptomyces strains, which interfere with the growth of A. Rugatob ispora selectively. Expand
Selective Isolation and Distribution ofSporichthya Strains in Soil
A simplified enrichment method in which centrifugation is used for selective isolation of Sporichthya strains from soil is described, finding that S. polymorpha was sensitive to 14 antibiotics, including nalidixic acid, and addition of these antibiotics was not suitable for isolating these strains. Expand
The lipA gene of Serratia marcescens which encodes an extracellular lipase having no N-terminal signal peptide
The lipA gene encoding an extracellular lipase was cloned from the wild-type strain of Serratia marcescens Sr41. Nucleotide sequencing showed a major open reading frame encoding a 64.9-kDa protein ofExpand
Boromycin, an anti-HIV antibiotic.
The mechanism for the anti-HIV activity of boromycin is suggested to involve blocking the later stage of HIV infection, and probably the maturity step for replication of the HIV molecule. Expand
Pathway for isoleucine formation form pyruvate by leucine biosynthetic enzymes in leucine-accumulating isoleucine revertants of Serratia marcescens.
Leaky revertants isolated from isoleucine auxotrophs of Serratia marcescens mutant resistant to alpha-aminobutyric acid are concluded to be synthesized from alpha-ketobutyrate via citramalate formed from pyruvate annd acetyl-coenzyme A by leucine biosynthetic enzymes, as a result of desensitization of alpha-isopropylmalate synthetase to feedback inhibition. Expand
Structures of TMC-95A-D: novel proteasome inhibitors from Apiospora montagnei sacc. TC 1093.
Four novel proteasome inhibitors, TMC-95A-D (1-4) have been isolated from the fermentation broth of Apiospora montagnei Sacc and the relationships of 1-4 to be diastereomers at C-7 and C-36 were revealed. Expand
Valine Accumulation by α-Aminobutyric Acid-Resistant Mutants of Serratia marcescens
α-Aminobutyric acid, norvaline, and norleucine, which are analogues of branched-chain amino acids, inhibited the growth of Serratia marcescens and a number of mutants resistant to these analogues were isolated. Expand