S. Kocabıyık

Publications27
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Citations164
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An extracellular--pepstatin insensitive acid protease produced by Thermoplasma volcanium.
In this study, some parameters for the production and caseinolytic activity of an extracellular thermostable acid protease from a thermoacidophilic archaeon Thermoplasma volcanium were determined.… Expand
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Intracellular alkaline proteases produced by thermoacidophiles: detection of protease heterogeneity by gelatin zymography and polymerase chain reaction (PCR).
In this study 24 thermoacidophilic archeal and bacterial strains isolated from hot-springs and hot-soils were screened for their ability to produce intracellular alkaline proteases. The protease… Expand
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Preparation of silica coated cobalt ferrite magnetic nanoparticles for the purification of histidine-tagged proteins
Abstract Surface modified cobalt ferrite (CoFe 2 O 4 ) nanoparticles containing Ni–NTA affinity group were synthesized and used for the separation of histidine tag proteins from the complex matrices… Expand
  • 20
Induced CYP1A mRNA, protein and catalytic activity in the liver of feral fish, leaping mullet, Liza saliens.
In this study, we examined whether levels of P4501A mRNA expression were naturally induced in feral fish, Liza saliens, and whether CYP1A protein levels and associated enzyme activity, EROD, were… Expand
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Essential structural and functional features of small heat shock proteins in molecular chaperoning process.
  • S. Kocabıyık
  • Chemistry, Medicine
  • Protein and peptide letters
  • 31 May 2009
Small heat shock proteins are ubiquitously found in all three domains of life, although they are the most poorly conserved family of molecular chaperones. Their involvement in anti-stress mechanisms… Expand
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Modified method for RNA isolation from bacteria by guanidinium thiocyanate-cesium chloride centrifugation.
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Molecular cloning and co-expression of Thermoplasma volcanium proteasome subunit genes.
In this study we describe, the construction of a co-expression vector allowing simultaneous production of Thermoplasma volcanium 20S proteasome alpha- and beta-subunits in Escherichia coli. This… Expand
  • 4
Purification and Characterization of an Intracellular Chymotrypsin-Like Serine Protease from Thermoplasma volcanium
An intracellular serine protease produced by Thermoplasma (Tp.) volcanium was purified using a combination of ammonium sulfate fractionation, ion exchange, and α-casein agarose affinity… Expand
  • 12
The effect of cysteine-43 mutation on thermostability and kinetic properties of citrate synthase from Thermoplasma acidophilum.
In this study, we have substituted serine-43 by cysteine in the recombinant citrate synthase from a moderately thermophilic Archaeon Thermoplasma acidophilum, for site-specific attachment of labels… Expand
  • 2
Cloning and overexpression of a thermostable signal peptide peptidase (SppA) from Thermoplasma volcanium GSS1 in E. coli.
In this study, a gene coding for thermophilic serine protease of the ClpP class from the thermoacidophilic archaeon Thermoplasma volcanium (Tpv) was cloned and expressed in Escherichia coli. The… Expand
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