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Crystal Structure of the Eukaryotic 60S Ribosomal Subunit in Complex with Initiation Factor 6
The 3.5 angstrom–resolution structure provides insights into the architecture of the eukaryotic ribosome and its regulation. Protein synthesis in all organisms is catalyzed by ribosomes. InExpand
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The complete structure of the small-subunit processome
The small-subunit processome represents the earliest stable precursor of the eukaryotic small ribosomal subunit. Here we present the cryo-EM structure of the Saccharomyces cerevisiae small-subunitExpand
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An iron-sulfur domain of the eukaryotic primase is essential for RNA primer synthesis
Primases synthesize the RNA primers that are necessary for replication of the parental DNA strands. Here we report that the heterodimeric archaeal/eukaryotic primase is an iron-sulfur (Fe-S) protein.Expand
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Mechanism for priming DNA synthesis by yeast DNA Polymerase α
The DNA Polymerase α (Pol α)/primase complex initiates DNA synthesis in eukaryotic replication. In the complex, Pol α and primase cooperate in the production of RNA-DNA oligonucleotides that primeExpand
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Shared Active Site Architecture between the Large Subunit of Eukaryotic Primase and DNA Photolyase
Background DNA synthesis during replication relies on RNA primers synthesised by the primase, a specialised DNA-dependent RNA polymerase that can initiate nucleic acid synthesis de novo. In archaealExpand
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Architecture of the yeast small subunit processome
A machine for building ribosomes The ribosome is a very large protein and RNA complex responsible for the difficult process of synthesizing proteins. Construction of the ribosome itself involvesExpand
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Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis
Eukaryotic ribosome biogenesis involves a plethora of ribosome-assembly factors, and their temporal order of association with preribosomal RNA is largely unknown. By using Saccharomyces cerevisiae asExpand
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Flexible tethering of primase and DNA Pol α in the eukaryotic primosome
The Pol α/primase complex or primosome is the primase/polymerase complex that initiates nucleic acid synthesis during eukaryotic replication. Within the primosome, the primase synthesizes short RNAExpand
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Atomic structures of the eukaryotic ribosome.
Eukaryotic ribosomes are significantly larger and more complex than their prokaryotic counterparts. This parallels the increased complexity of the associated cellular machinery responsible forExpand
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Crystal structure of the yeast ribosomal protein rpS3 in complex with its chaperone Yar1.
Eukaryotic ribosome assembly involves a plethora of factors, which ensure that a correctly folded ribosome contains all ribosomal protein components. Among these assembly factors, Yar1 has recentlyExpand
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