Culture of Preimplantation Mouse Embryos Affects Fetal Development and the Expression of Imprinted Genes1
- S. Khosla, W. Dean, David Brown, W. Reik, R. Feil
- BiologyBiology of Reproduction
- 1 March 2001
Whether culture of preimplantation mouse embryos in a chemically defined medium with or without fetal calf serum (FCS) can affect their subsequent development and imprinted gene expression is determined.
Culture of preimplantation embryos and its long-term effects on gene expression and phenotype.
A review of currently available data on aberrant offspring development that sometimes arises from commonly applied in-vitro procedures in humans, ruminant species and mice shows support for the epigenetic deregulation of developmentally important genes in culture.
The DNA methyltranferase Dnmt2 participates in RNA processing during cellular stress
- D. Thiagarajan, R. Dev, S. Khosla
- BiologyEpigenetics
- 1 January 2011
It is shown that Dnmt2 is a part of RNA processing machinery during cellular stress and exhibits nucleo-cytoplasmic shuttling in response to cellular stress, and could represent a primitive cellular defense mechanism against viral infection.
A novel nucleoid-associated protein of Mycobacterium tuberculosis is a sequence homolog of GroEL
- Debashree Basu, Garima Khare, Shashi Singh, A. Tyagi, S. Khosla, S. Mande
- BiologyNucleic Acids Research
- 15 June 2009
Findings reveal that M. tuberculosis GroEL1 has evolved to be associated with nucleoids and can effectively function as a DNA-protecting agent against DNase I or hydroxyl-radicals.
The interaction of mycobacterial protein Rv2966c with host chromatin is mediated through non-CpG methylation and histone H3/H4 binding
- G. Sharma, S. Upadhyay, M. Srilalitha, V. Nandicoori, S. Khosla
- BiologyNucleic Acids Research
- 30 March 2015
This is the first study that identifies a protein from a pathogenic bacteria with potential to influence host DNA methylation in a non-canonical manner providing the pathogen with a novel mechanism to alter the host epigenetic machinery.
Genomic imprinting in ruminants: allele-specific gene expression in parthenogenetic sheep
It is demonstrated that, like in mice and humans, the growth-related PEG1/MEST and Insulin-like Growth Factor 2 (IGF2) genes are expressed from the paternal chromosome in sheep, suggesting that genomic imprinting is conserved in a third, evolutionarily rather diverged group of placental mammals, the ruminants.
DNA Methylation Is Linked to Deacetylation of Histone H3, but Not H4, on the Imprinted Genes Snrpnand U2af1-rs1
- R. Gregory, Tamzin E Randall, R. Feil
- Biology, ChemistryMolecular and Cellular Biology
- 15 August 2001
Data from ChIP and resolution of parental alleles using single-strand conformational polymorphisms are consistent with the hypothesis that CpG methylation leads to deacetylation of histone H3, but not H4, through a process that involves selective binding of MBD proteins.
Genomic imprinting in the mealybugs
- S. Khosla, G. Mendiratta, V. Brahmachari
- BiologyCytogenetic and Genome Research
- 1 March 2006
The mealybug system may provide evidence for stable maintenance of chromatin code not only through mitosis but also through meiosis, in light of the accruing experimental evidence for chromatin-based differences in the maternal and paternal genomes.
Parental Allele-Specific Chromatin Configuration in a Boundary–Imprinting-Control Element Upstream of the MouseH19 Gene
- S. Khosla, A. Aitchison, R. Gregory, N. Allen, R. Feil
- BiologyMolecular and Cellular Biology
- 1 April 1999
The data suggest that nonhistone proteins are associated with the maternal chromosome and that these might be involved in its boundary function, and the chromatin organization and nucleosomal organization of this element are mutually exclusive and reflect alternate epigenetic states.
Mycobacteria modulate host epigenetic machinery by Rv1988 methylation of a non-tail arginine of histone H3.
- Imtiyaz Yaseen, Prabhjot Kaur, V. Nandicoori, S. Khosla
- BiologyNature Communications
- 16 November 2015
It is shown that Rv1988, a secreted mycobacterial protein, is a functional methyltransferase that localizes to the host nucleus and interacts with chromatin that methylates histone H3 at H3R42 and represses the genes involved in the first line of defence againstMycobacteria.
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