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The conserved kinetochore protein shugoshin protects centromeric cohesion during meiosis
TLDR
Sgo1 (shugoshin), a protector of the centromeric cohesin Rec8 in fission yeast, and a homologue of Sgo1 in budding yeast are identified, providing insights into the evolution of meiosis and kinetochore regulation during mitosis and meiosis.
Phosphorylation of H2A by Bub1 Prevents Chromosomal Instability Through Localizing Shugoshin
TLDR
It is shown that Bub1 phosphorylates the conserved serine 121 of histone H2A in fission yeast Schizosaccharomyces pombe, which creates a mark for shugoshin localization and the correct partitioning of chromosomes.
Shugoshin collaborates with protein phosphatase 2A to protect cohesin
TLDR
A conserved mechanism of centromeric protection of eukaryotic chromosomes in mitosis and meiosis is revealed by finding a specific subtype of serine/threonine protein phosphatase 2A (PP2A) associating with human shugoshin.
Shugoshin enables tension-generating attachment of kinetochores by loading Aurora to centromeres.
TLDR
It is demonstrated that, unlike Sgo1, Sgo2 is dispensable for centromeric protection of cohesin and interacts with Bir1/Survivin and promotes Aurora kinase complex localization to the pericentromeric region, to correct erroneous attachment of kinetochores and enable tension-generating attachment.
Aurora controls sister kinetochore mono-orientation and homolog bi-orientation in meiosis-I
TLDR
The results provide an explanation for the previously enigmatic observation that fission yeast Shugoshin Sgo2, which assists in loading Aurora to centromeres, and its regulator Bub1 are required for the mono‐orientation of sister chromatids in meiosis‐I.
Optimization of the analogue-sensitive Cdc2/Cdk1 mutant by in vivo selection eliminates physiological limitations to its use in cell cycle analysis
TLDR
In vivo selection is used for intragenic suppressor mutations of cdc2-as that restore full function in the absence of ATP-analogues and show that Cdc2 activity is required to maintain the activity of the spindle assembly checkpoint, and it is demonstrated that maintenance of the Shugoshin Sgo1 at meiotic centromeres does not require CDC2 activity, whereas localization of the kinase aurora does.
Dissecting the first and the second meiotic divisions using a marker-less drug-hypersensitive fission yeast
TLDR
A powerful strategy to dissect meiosis I and meiosis II, using chemical inhibitors in genetically tractable model organism fission yeast, and examining Aurora-dependent spindle assembly checkpoint (SAC) regulation during meiosis found that Aurora B/Ark1 kinase activity is required for recruitment of Bub1, an essential SAC kinase, to unattached kinetochore in prometaphase I and prometAPHase II as in mitosis.
Nuclear envelope expansion is crucial for proper chromosomal segregation during a closed mitosis
TLDR
Identification of a new fatty acid synthase inhibitor for nuclear division by a chemical genetic screen revealed a link between nuclear envelope expansion and faithful chromosome segregation in a closed mitosis.
Examining post‐translational modification‐mediated protein–protein interactions using a chemical proteomics approach
TLDR
ClASPI can be used to analyze methylation‐dependent protein–protein interactions in lysates of fission yeast, a genetically tractable model organism and demonstrate that the CLASPI approach can be broadly applied to profile protein– protein interactions mediated by PTMs.
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