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Investigation of the Structural Basis for Thermostability of DNA-binding Protein HU from Bacillus stearothermophilus *
Site-directed mutagenesis was used to identify amino acid residues essential for the thermostability of the DNA-binding protein HU from the thermophile Bacillus stearothermophilus (BstHU). TwoExpand
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Experimental verification of the crucial roles of Glu73 in the catalytic activity and structural stability of goose type lysozyme.
The roles of Glu(73), which has been proposed to be a catalytic residue of goose type (G-type) lysozyme based on X-ray structural studies, were investigated by means of its replacement with Gln, Asp,Expand
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Catalytic reaction mechanism of goose egg-white lysozyme by molecular modelling of enzyme-substrate complex.
Despite the low similarity between their amino acid sequences, the core structures of the fold between chicken-type and goose-type lysozymes are conserved. However, their enzymatic activities areExpand
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Arginine-55 in the beta-arm is essential for the activity of DNA-binding protein HU from Bacillus stearothermophilus.
DNA-binding protein HU (BstHU) from Bacillus stearothermophilus is a homodimeric protein which binds to DNA in a sequence-nonspecific manner. In order to identify the Arg residues essential for DNAExpand
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Glycine-15 in the bend between two alpha-helices can explain the thermostability of DNA binding protein HU from Bacillus stearothermophilus.
On the basis of sequence comparison of thermophilic and mesophilic DNA binding protein HUs, Bacillus stearothermophilus DNA binding protein HU (BstHU) seems to gain thermostability with a change inExpand
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Contribution of a salt bridge to the thermostability of DNA binding protein HU from Bacillus stearothermophilus determined by site-directed mutagenesis.
We have employed site-directed mutagenesis to evaluate the contribution of the amino acid residues, Glu34, Arg37, and Lys38, to the thermostability of the thermophilic protein, BacillusExpand
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Construction of enzyme-substrate complexes between hen egg-white lysozyme and N-acetyl-D-glucosamine hexamer by systematic conformational search and molecular dynamics simulation.
We constructed the complexes between HEWL and (GlcNAc)6 oligomer in order to investigate the amino acid residues related to substrate binding in the productive and nonproductive complexes, and theExpand
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Complete amino acid sequence of three reptile lysozymes.
To study the structure and function of reptile lysozymes, we have reported their purification, and in this study we have established the amino acid sequence of three egg white lysozymes inExpand
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Role of disulfide bonds in goose‐type lysozyme
The role of the two disulfide bonds (Cys4–Cys60 and Cys18–Cys29) in the activity and stability of goose‐type (G‐type) lysozyme was investigated using ostrich egg‐white lysozyme as a model. Each ofExpand
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Molecular mechanism of S-modulin action: binding target and effect of ATP.
S-Modulin is suggested to increase the light sensitivity of rods by inhibiting phosphorylation of light-activated rhodopsin (Rh*) at high Ca2+ concentrations. The inhibition of rhodopsinExpand
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