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Molecular characterization of Escherichia coli NAD kinase.
NAD kinase was purified to homogeneity from Escherichia coli MG1655. The enzyme was a hexamer consisting of 30 kDa subunits and utilized ATP or other nucleoside triphosphates as phosphoryl donors forExpand
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Structure and Function of NAD Kinase and NADP Phosphatase: Key Enzymes That Regulate the Intracellular Balance of NAD(H) and NADP(H)
  • S. Kawai, K. Murata
  • Biology, Medicine
  • Bioscience, biotechnology, and biochemistry
  • 23 April 2008
The functions of NAD(H) (NAD+ and NADH) and NADP(H) (NADP+ and NADPH) are undoubtedly significant and distinct. Hence, regulation of the intracellular balance of NAD(H) and NADP(H) is important. TheExpand
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Degradation mechanisms of phenolic beta-1 lignin substructure model compounds by laccase of Coriolus versicolor.
Phenolic beta-1 lignin substructure model compounds, 1-(3,5-dimethoxy-4-hydroxy-phenyl)-2-(3,5-dimethoxy-4-ethoxyphenyl)propa ne-1, 3-diol (I) and 1-(3,5-dimethoxy-4-ethoxyphenyl)-2-(3,Expand
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Molecular cloning and identification of UTR1 of a yeast Saccharomyces cerevisiae as a gene encoding an NAD kinase.
UTR1 of the yeast Saccharomyces cerevisiae was cloned from the genomic DNA by polymerase chain reaction and expressed in Escherichia coli. Characterization of the purified UTR1p revealed that UTR1pExpand
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Transformation of Saccharomyces cerevisiae and other fungi
Transformation (i.e. genetic modification of a cell by the incorporation of exogenous DNA) is indispensable for manipulating fungi. Here, we review the transformation methods for SaccharomycesExpand
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Characterization and Molecular Cloning of a Novel Enzyme, Inorganic Polyphosphate/ATP-Glucomannokinase, of Arthrobacter sp. Strain KM
ABSTRACT A bacterium exhibiting activities of several inorganic polyphosphate [poly(P)]- and ATP-dependent kinases, including glucokinase, NAD kinase, mannokinase, and fructokinase, was isolated,Expand
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Two Sources of Mitochondrial NADPH in the Yeast Saccharomyces cerevisiae*
Cells of the yeast Saccharomyces cerevisiae contain three NAD kinases; namely, cytosolic Utr1p, cytosolic Yef1p, and mitochondrial Pos5p. Previously, the NADH kinase reaction catalyzed by Pos5p,Expand
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Inorganic Polyphosphate/ATP-NAD kinase of Micrococcus flavus and Mycobacterium tuberculosis H37Rv.
An enzyme with both inorganic polyphosphate [poly(P)]- and ATP-dependent NAD kinase activities was isolated from Micrococcus flavus. The enzyme was a dimer consisting of 34 kDa subunits, and wasExpand
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Identification of ATP‐NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae
ATP‐NAD kinase phosphorylates NAD to produce NADP by using ATP, whereas ATP‐NADH kinase phosphorylates both NAD and NADH. Three NAD kinase homologues, namely, ATP‐NAD kinase (Utr1p), ATP‐NADH kinaseExpand
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Molecular Conversion of NAD Kinase to NADH Kinase through Single Amino Acid Residue Substitution*
NAD kinase phosphorylates NAD+ to form NADP+ and is strictly specific to NAD+, whereas NADH kinase phosphorylates both NAD+ and NADH, thereby showing relaxed substrate specificity. Based on theirExpand
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