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Phosphate-binding pocket in Dicer-2 PAZ domain for high-fidelity siRNA production
- S. Kandasamy, R. Fukunaga
- Biology, Medicine
- Proceedings of the National Academy of Sciences
- 21 November 2016
It is proposed that the 5′-monophosphate of a long dsRNA substrate is anchored by the phosphate-binding pocket in the Dicer-2 PAZ domain and the distance between the pocket and the RNA cleavage active site in the RNaseIII domain corresponds to the 21-nt pitch in the A-form duplex of along ds RNA substrate, resulting in high-fidelity 21-NT siRNA production. Expand
Dicer partner protein tunes the length of miRNAs using base-mismatch in the pre-miRNA stem
Using Dram-seq, a novel high-throughput method, it is found that a base-mismatch in the pre-miRNA stem can alter the length of miRNAs compared with abase-pair at the same position in both Drosophila and human, and is important for the miRNA length tuning by Loqs-PB. Expand
LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes
- Li Zhu, S. Kandasamy, Susan E Liao, R. Fukunaga
- Chemistry, Medicine
- Nature Communications
- 2 October 2018
This study identifies MARF1 as a regulator in oocyte maturation and defines the conserved LOTUS domain as a post-transcriptional effector domain that recruits CCR4-NOT deadenylase complex to shorten target mRNA poly-A tails and suppress their translation during Drosophila oogenesis. Expand
DEAD-box RNA helicase Belle posttranscriptionally promotes gene expression in an ATPase activity-dependent manner.
Systematic mutational analysis found that Belle RNA-binding and RNA-unwinding activities and intrinsically disordered regions (IDRs) are required for Belle in vivo function and proposed that Belle promotes translation of specific target mRNAs, including cyclin B mRNA, in an ATPase activity-dependent manner. Expand
The C-terminal dsRNA-binding domain of Drosophila Dicer-2 is crucial for efficient and high-fidelity production of siRNA and loading of siRNA to Argonaute2.
Light is shed on the molecular mechanism by which Dicer-2 produces 21-nt siRNAs with a high efficiency and fidelity for efficient RNA silencing and proposes that CdsRBD binds the proximal body region of a long dsRNA substrate whose 5'-monophosphate end is anchored by the phosphate-binding pocket in the PAZ domain. Expand
Common miR-590 Variant rs6971711 Present Only in African Americans Reduces miR-590 Biogenesis
SNP-induced reduction in miR-590 biogenesis could influence cardiac phenotype by de-repression of these signaling pathways, and population studies in this patient population would be valuable to investigate effects of this SNP on myocyte function and cardiac physiology. Expand
Author Correction: LOTUS domain protein MARF1 binds CCR4-NOT deadenylase complex to post-transcriptionally regulate gene expression in oocytes
The originally published version of this Article contained an error in Figure 1a, in which the length of the protein fragment produced by the MARF1 null allele was incorrectly labelled as ‘34aa’… Expand