Share This Author
Scale-down simulators for metabolic analysis of large-scale bioprocesses.
High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactor
- Julia Glazyrina, Eva-Maria Materne, P. Neubauer
- Engineering, BiologyMicrobial cell factories
- 30 May 2010
Rocking-motion-type bioreactors may provide an interesting alternative to standard cultivation in bioreacts for cultivation of bacteria and recombinant protein production and internal substrate delivery systems such as EnBase® Flo provide an opportunity for adopting simple to use fed-batch-type strategies to shaken cultures.
A two-compartment bioreactor system made of commercial parts for bioprocess scale-down studies: impact of oscillations on Bacillus subtilis fed-batch cultivations.
- S. Junne, A. Klingner, Johannes Kabisch, T. Schweder, P. Neubauer
- Engineering, BiologyBiotechnology journal
- 1 August 2011
An advanced version of a two-compartment scale-down bioreactor that simulates inhomogeneities present in large-scale industrial bioreactors on the laboratory scale is described, found that oscillating substrate and DO concentration led to diminished glucose uptake, ethanol formation and an altered amino acid synthesis.
Response of Corynebacterium glutamicum exposed to oscillating cultivation conditions in a two‐ and a novel three‐compartment scale‐down bioreactor
- Anja Lemoine, Nina Maya Martίnez-Iturralde, R. Spann, P. Neubauer, S. Junne
- Biology, EngineeringBiotechnology and bioengineering
- 1 June 2015
Although the strain shows robustness, the product yield of lysine is reduced in scale‐down cultivations as compared to cultivations at homogeneous conditions, which underlines the relevance of process optimization.
Antisense RNA Downregulation of Coenzyme A Transferase Combined with Alcohol-Aldehyde Dehydrogenase Overexpression Leads to Predominantly Alcohologenic Clostridium acetobutylicum Fermentations
DNA microarray analysis showed that aad overexpression profoundly affects the large-scale transcriptional program of the cells, and the expression patterns of the CoAT genes (ctfA and ctfB) and aad were consistent with the overeexpression of aad and asRNA downregulation of cTFB.
Consistent development of bioprocesses from microliter cultures to the industrial scale
The paper reviews the bioprocess developmental phases from a business perspective and the available systems and technologies.
Process inhomogeneity leads to rapid side product turnover in cultivation of Corynebacterium glutamicum
- Friedrich Käß, S. Junne, P. Neubauer, W. Wiechert, M. Oldiges
- Biology, EngineeringMicrobial Cell Factories
- 10 January 2014
The metabolic phenotype of C. glutamicum seems to be particularly well-suited for cultivation at inhomogeneous process conditions for large-scale fed-batch application, which is in good accordance with the respective industrial experiences.
Assessment of robustness against dissolved oxygen/substrate oscillations for C. glutamicum DM1933 in two-compartment bioreactor
- Friedrich Käß, Ioanna Hariskos, M. Oldiges
- Biology, EngineeringBioprocess and Biosystems Engineering
- 1 June 2014
Microbial physiology of C.glutamicum appears to be ideally adapted to both homogeneous and inhomogeneous conditions, which ensures exceptional suitability for cultivation at increased mixing times, which is suggested to constitute an important basis for the long-lasting success in large scale bioprocess application.
Transcriptional analysis of product‐concentration driven changes in cellular programs of recombinant Clostridium acetobutylicumstrains
- S. Tummala, S. Junne, C. Paredes, E. Papoutsakis
- BiologyBiotechnology and bioengineering
- 30 December 2003
Cluster analysis and gene expression patterns of established and putative operons involved in stress response, motility, sporulation, and fatty‐acid biosynthesis indicate that these simple genetic changes dramatically alter the cellular programs of C. acetobutylicum.
Anaerobic Digestion Model (AM2) for the Description of Biogas Processes at Dynamic Feedstock Loading Rates
The anaerobic digestion of biomass is a complex, dynamic, and highly nonlinear process. Hence, a mathematical model that is capable of describing the complete operability region with sufficient…