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Genetic transformation of Tylophora indica with Agrobacterium rhizogenes A4: growth and tylophorine productivity in different transformed root clones
We have developed an efficient transformation system for Tylophora indica, an important medicinal plant in India, using Agrobacterium rhizogenes strains LBA9402 and A4 to infect excised leaf and stemExpand
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Withanolide synthesis in cultures of Withania somnifera transformed with Agrobacterium tumefaciens
Abstract Transformed organ cultures of Withania somnifera were established following infection with wild type nopaline and octopine strains of Agrobacterium tumefaciens. The oncogenic strains hadExpand
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In vitro propagation of cashewnut
In vitro plant propagation was developed for seedling shoot tips, leaf axils, and cotyledonary nodes of cashew, Anacardium occidentale. Factors affecting multiplication rate included age of explantExpand
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Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera
Developmental variability was introduced into Withania somnifera using genetic transformation by Agrobacterium rhizogenes, with the aim of changing withasteroid production. Inoculation of W.Expand
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The root: a potential new source of competent cells for high-frequency regeneration in Tylophora indica
The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material. Root explants cultured onExpand
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Somatic embryogenesis from immature cotyledons of an elite Darjeeling tea clone
Abstract Cotyledons from immature embryos of Camellia sinensis O. Kuntze var. T-78, an elite Darjeeling tea clone, were exposed to various levels of 2,4-D, NAA, IBA, BAP and Kin (1–10 mg/1). While noExpand
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Improved taxol yield in cell suspension culture of Taxus wallichiana (Himalayan yew)
Cell culture of Taxus wallichiana Zucc (= T. baccata ssp wallichiana Zucc. Pilg.) (Himalayan yew) has been established and taxol-producing cell lines selected by cell line cloning. Cell line NC110Expand
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Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium
We examined the effects of genetic transformation by Agrobacterium rhizogenes on the production of tylophorine, a phenanthroindolizidine alkaloid, in the Indian medicinal plant, Tylophora indica.Expand
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Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants
We have developed an efficient transformation system for Bacopa monnieri, an important Indian medicinal plant, using Agrobacterium rhizogenes strains LBA 9402 and A4. Transformed roots induced byExpand
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Organogenesis and plant regeneration in Taxus wallichiana (Zucc.)
We describe an efficient process for regeneration of Taxus wallichiana plants via shoot organogenesis from callus cultures derived from zygotic embryos. Zygotic embryos cultured on half strengthExpand
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