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Genetic transformation of Tylophora indica with Agrobacterium rhizogenes A4: growth and tylophorine productivity in different transformed root clones
An efficient transformation system for Tylophora indica is developed, using Agrobacterium rhizogenes strains LBA9402 and A4 to infect excised leaf and stem explants and intact shoots at different sites to induce transformed roots.
The root: a potential new source of competent cells for high-frequency regeneration in Tylophora indica
A new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material was developed, with MS medium supplemented with 10.72 μM BA being the most effective in inducing FEC and somatic embryogenesis.
In vitro propagation of cashewnut
In vitro plant propagation was developed for seedling shoot tips, leaf axils, and cotyledonary nodes of cashew, Anacardium occidentale and the highest frequency of rooted shoots was obtained by treating shoots with the bacterium, Agrobacterium rhizogenes.
Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera
The first report of the presence of withasteroids in undifferentiated callus cultures of W. somnifera is reported, and this is the first detection of withaferin A in the roots of the plant.
Withanolide synthesis in cultures of Withania somnifera transformed with Agrobacterium tumefaciens
Improved taxol yield in cell suspension culture of Taxus wallichiana (Himalayan yew)
Using three different cell lines with different taxol-producing capacities, it has been demonstrated that 2,4-D and IAA-phenylalanine when present alone favour growth and taxol production but when combined enhance biomass to a maximum (six-fold in NC110) without enhancing taxol accumulation, suggesting that a two-stage culture may be beneficial for optimising taxol collection in cell culture of T. wallichiana.
Somatic embryogenesis from immature cotyledons of an elite Darjeeling tea clone
Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures
This is a comprehensive review about the progress in the elicitation approach to hairy root cultures from 2010 to 2019 and the information provided is valuable and will be of interest for scientists working in this area of plant biotechnology.
Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium…
Transformed roots induced by the bacterium grew in axenic culture and produced shoots or embryogenic calli in the absence of hormone treatments, however, hormonal treatment was required to regenerate shoots in root explants of wild type control plants.
Organogenesis and plant regeneration in Taxus wallichiana (Zucc.)
We describe an efficient process for regeneration of Taxus wallichiana plants via shoot organogenesis from callus cultures derived from zygotic embryos. Zygotic embryos cultured on half strength…