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Genetic transformation of Tylophora indica with Agrobacterium rhizogenes A4: growth and tylophorine productivity in different transformed root clones
- K. Chaudhuri, B. Ghosh, D. Tepfer, S. Jha
- Biology, Medicine
- Plant Cell Reports
- 20 January 2005
We have developed an efficient transformation system for Tylophora indica, an important medicinal plant in India, using Agrobacterium rhizogenes strains LBA9402 and A4 to infect excised leaf and stem… Expand
Withanolide synthesis in cultures of Withania somnifera transformed with Agrobacterium tumefaciens
Abstract Transformed organ cultures of Withania somnifera were established following infection with wild type nopaline and octopine strains of Agrobacterium tumefaciens. The oncogenic strains had… Expand
In vitro propagation of cashewnut
In vitro plant propagation was developed for seedling shoot tips, leaf axils, and cotyledonary nodes of cashew, Anacardium occidentale. Factors affecting multiplication rate included age of explant… Expand
Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera
- M. Bandyopadhyay, S. Jha, D. Tepfer
- Biology, Medicine
- Plant Cell Reports
- 16 April 2007
Developmental variability was introduced into Withania somnifera using genetic transformation by Agrobacterium rhizogenes, with the aim of changing withasteroid production. Inoculation of W.… Expand
The root: a potential new source of competent cells for high-frequency regeneration in Tylophora indica
- K. Chaudhuri, B. Ghosh, S. Jha
- Biology, Medicine
- Plant Cell Reports
- 13 February 2004
The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material. Root explants cultured on… Expand
Somatic embryogenesis from immature cotyledons of an elite Darjeeling tea clone
Abstract Cotyledons from immature embryos of Camellia sinensis O. Kuntze var. T-78, an elite Darjeeling tea clone, were exposed to various levels of 2,4-D, NAA, IBA, BAP and Kin (1–10 mg/1). While no… Expand
Improved taxol yield in cell suspension culture of Taxus wallichiana (Himalayan yew)
Cell culture of Taxus wallichiana Zucc (= T. baccata ssp wallichiana Zucc. Pilg.) (Himalayan yew) has been established and taxol-producing cell lines selected by cell line cloning. Cell line NC110… Expand
Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium…
- K. Chaudhuri, B. Ghosh, D. Tepfer, S. Jha
- Biology, Medicine
- Plant Cell Reports
- 12 April 2006
We examined the effects of genetic transformation by Agrobacterium rhizogenes on the production of tylophorine, a phenanthroindolizidine alkaloid, in the Indian medicinal plant, Tylophora indica.… Expand
Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants
- S. Majumdar, S. Garai, S. Jha
- Biology, Medicine
- Plant Cell Reports
- 25 February 2011
We have developed an efficient transformation system for Bacopa monnieri, an important Indian medicinal plant, using Agrobacterium rhizogenes strains LBA 9402 and A4. Transformed roots induced by… Expand
Organogenesis and plant regeneration in Taxus wallichiana (Zucc.)
- M. Datta, A. Majumder, S. Jha
- Biology, Medicine
- Plant Cell Reports
- 1 February 2006
We describe an efficient process for regeneration of Taxus wallichiana plants via shoot organogenesis from callus cultures derived from zygotic embryos. Zygotic embryos cultured on half strength… Expand
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