• Publications
  • Influence
ATM-Dependent Chromatin Changes Silence Transcription In cis to DNA Double-Strand Breaks
DNA double-strand breaks (DSBs) initiate extensive local and global alterations in chromatin structure, many of which depend on the ATM kinase. Histone H2A ubiquitylation (uH2A) on chromatinExpand
  • 523
  • 54
  • PDF
From Silencing to Gene Expression Real-Time Analysis in Single Cells
We have developed an inducible system to visualize gene expression at the levels of DNA, RNA and protein in living cells. The system is composed of a 200 copy transgene array integrated into aExpand
  • 629
  • 28
  • PDF
Dynamics of Single mRNPs in Nuclei of Living Cells
Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA)expression to characterize the movementExpand
  • 472
  • 21
  • PDF
The ACF1 complex is required for DNA double-strand break repair in human cells.
DNA double-strand breaks (DSBs) are repaired via nonhomologous end-joining (NHEJ) or homologous recombination (HR), but cellular repair processes remain elusive. We show here that the ATP-dependentExpand
  • 184
  • 19
A Myristoylated Calcium-binding Protein that Preferentially Interacts with the Alzheimer's Disease Presenilin 2 Protein
It is well established that mutations in the presenilin 1 and 2 genes cause the majority of early onset Alzheimer's disease (AD). However, our understanding of the cellular functions of the proteinsExpand
  • 131
  • 14
  • PDF
Visualization of gene activity in living cells
Chromatin structure is thought to play a critical role in gene expression. Using the lac operator/repressor system and two colour variants of green fluorescent protein (GFP), we developed a system toExpand
  • 300
  • 11
Metabolic-energy-dependent movement of PML bodies within the mammalian cell nucleus
Promyelocytic leukaemia (PML) nuclear bodies are present in most mammalian cell nuclei. PML bodies are disrupted by PML retinoic acid receptor alpha (RARα) oncoproteins in acute promyelocyticExpand
  • 188
  • 8
The ATM Substrate KAP1 Controls DNA Repair in Heterochromatin: Regulation by HP1 Proteins and Serine 473/824 Phosphorylation
The repair of DNA damage in highly compact, transcriptionally silent heterochromatin requires that repair and chromatin packaging machineries be tightly coupled and regulated. KAP1 is aExpand
  • 92
  • 8
  • PDF
Single Cell Analysis of Transcriptional Activation Dynamics
Background Gene activation is thought to occur through a series of temporally defined regulatory steps. However, this process has not been completely evaluated in single living mammalian cells.Expand
  • 36
  • 5
  • PDF
Increased Apoptosis Arising from Increased Expression of the Alzheimer's Disease–associated Presenilin-2 Mutation (N141I)
Mutations in the genes for presenilin 1 and 2 (PS-1 and PS-2) have been linked to development of early-onset Alzheimer's disease (AD). As neither the normal function of either presenilin is known norExpand
  • 118
  • 3
  • PDF