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Asparaginyl endopeptidase of jack bean seeds. Purification, characterization, and high utility in protein sequence analysis.
Strict substrate specificity of this enzyme was verified by the use of 14 polypeptide substrates including those derived from proteins, and behavior of the enzyme toward various protease inhibitors suggested that it belongs to a family of cysteine proteases. Expand
Halocyamines: novel antimicrobial tetrapeptide-like substances isolated from the hemocytes of the solitary ascidian Halocynthia roretzi.
Two novel antimicrobial tetrapeptide-like substances, halocyamine A and B, were isolated from the solitary ascidian Halocynthia roretzi by a procedure including extraction steps, chromatographies onExpand
Purification and characterization of multicatalytic proteinase from eggs of the ascidian Halocynthia roretzi.
The properties of the enzyme purified from ascidian eggs are similar to those of multicatalytic proteinases from mammalian tissues. Expand
Isolation and analysis of cDNA encoding a precursor of Canavalia ensiformis asparaginyl endopeptidase (legumain).
This may be the first report, however, that deals with the primary structure of such a proteinase, and the deduced amino acid sequences of these enzyme precursors were compared with those in the GeneBank, EMBL, and NBRF databases. Expand
Nucleotide sequence of the tail tube structural gene of bacteriophage T4.
The nucleotide sequence of gene 19 of bacteriophage T4, the structural gene of the tail tube protein, was determined by both the dideoxy and the Maxam-Gilbert methods, and the implication was examined by an S1 nuclease protection experiment. Expand
Primary structure of Streptomyces griseus metalloendopeptidase II.
Based on the sequence comparison of SGMPII and other bacterial metalloproteases, the structural basis for the differences in substrate specificity and stability between SGMP II and other thermolysin-like proteases is discussed. Expand
Purification and fluorometric assay of proteinase A from yeast.
Proteinase A was purified from commercial baker's yeast to homogeneity by using affinity chromatography. Simple and sensitive fluorometric assay procedures were developed for this enzyme, whereExpand
A new feature of angiotensin-converting enzyme in the brain: hydrolysis of substance P.
The analyses of cleavage products indicate that the enzyme hydrolyzes substance P between Phe7-Phe8 and Phe8-Gly9 by an endopeptidase action, followed by successive release of dipeptides by a di peptidyl carboxypeptid enzyme action. Expand
Frontal affinity chromatography: theory for its application to studies on specific interactions of biomolecules.
Frontal analysis in affinity chromatography provides information almost identical to that obtainable by enzyme kinetic studies, especially for complicated systems where it has been difficult to find an appropriate method. Expand