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Structural and mechanistic insights into the bacterial amyloid secretion channel CsgG
Structural, functional and electrophysiological analyses imply that Escherichia coli CsgG is an ungated, non-selective protein secretion channel that is expected to employ a diffusion-based, entropy-driven transport mechanism. Expand
Sequence-specific detection of individual DNA strands using engineered nanopores
Biosensor elements that are capable of identifying individual DNA strands with single-base resolution are described, exemplified by the detection of a drug resistance–conferring mutation in the reverse transcriptase gene of HIV. Expand
Nanopore analytics: sensing of single molecules.
In nanopore analytics, individual molecules pass through a single nanopore giving rise to detectable temporary blockades in ionic pore current, which ranges from nucleic acids, peptides, proteins, and biomolecular complexes to organic polymers and small molecules. Expand
Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore
A new type of biosensor element for detecting proteins in solution at nanomolar concentrations tethered to the lumen of the transmembrane protein pore allows detection of a protein analyte at the single-molecule level, facilitating both quantification and identification through a distinctive current signature. Expand
Kinetics of duplex formation for individual DNA strands within a single protein nanopore
Details of the kinetics difficult to obtain by conventional methods, like surface plasmon resonance, which measure ensemble properties are revealed by investigating the temperature dependence of DNA duplex formation at the single molecule level. Expand
Improved Kelvin probe force microscopy for imaging individual DNA molecules on insulating surfaces
Electrostatic forces and potentials are keys in determining the interactions between biomolecules. We have recently imaged the topography and electrostatic surface potential of nucleic acid moleculesExpand
Self-assembled DNA nanopores that span lipid bilayers.
It is expected that the small membrane-spanning DNA pore will help open up the design of entirely new molecular devices for a broad range of applications including sensing, electric circuits, catalysis, and research into nanofluidics and controlled transmembrane transport. Expand
SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer assembly
The SbsB lattice represents a previously undescribed structural model for protein assemblies and may advance the understanding of SLP physiology and self-assembly, as well as the rational design of engineered higher-order structures for biotechnology. Expand