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Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand
A facile approach for designing families of GPCRs with engineered ligand specificities will prove to be powerful tools for selectively modulating signal-transduction pathways in vitro and in vivo.
PIP2 and PIP as determinants for ATP inhibition of KATP channels.
It is reported here that phosphatidylinositol-4, 5-bisphosphate (PIP2) and phosphorus-4-phosphates(PIP) controlled ATP inhibition of cloned KATP channels (Kir6.2 and SUR1) and represents a mechanism for control of excitability through phospholipids.
Modulation of Ca2+ channels by G-protein βγ subunits
For the top panel of Fig. Id, GTP-yS is also included in the intracellular solution as stated in the figure legend, and not GDP- yS as indicated on the figure itself.
A genetic approach to access serotonin neurons for in vivo and in vitro studies.
A transgenic route to selectively alter 5HT neuron gene expression is presented based on the ability of a Pet-1 enhancer region to direct reliable 5 HT neuron-specific transgene expression in the CNS and establishes a straightforward and reliable approach for developing an array of tools for in vivo and in vitro studies of 5HT neurons.
Fast noninvasive activation and inhibition of neural and network activity by vertebrate rhodopsin and green algae channelrhodopsin.
Light-activated vertebrate RO4 and green algae ChR2 allow the antagonistic control of neuronal function within ms to s in a precise, reversible, and noninvasive manner in cultured neurons and intact vertebrate spinal cords.
Modulation of Ca2+ channels βγ G-protein py subunits
CALCIUM ions entering cells through voltage-gated Ca2+ channels initiate rapid release of neurotransmitters and secretion of hormones. Ca2+ currents can be inhibited in many cell types by
Molecular determinants of inactivation and G protein modulation in the intracellular loop connecting domains I and II of the calcium channel alpha1A subunit.
Results indicate that the QXXER motif in L I-II is an important determinant of both voltage-dependent inactivation and G protein modulation, and that the amino acid in the third position of this motif has an unexpectedly large influence on modulation by Gbetagamma.
Pet-1 is required across different stages of life to regulate serotonergic function
It is found that Pet-1 was still required in adult 5-HT neurons to preserve normal anxiety-related behaviors through direct autoregulated control of serotonergic gene expression, indicating that behavioral pathogenesis can result from both developmental and adult-onset alterations in Serotonergic transcription.
Modulation of Ca2+ channels by G-protein beta gamma subunits.
It is reported that G beta gamma subunits can modulate Ca2+ channels and that overexpression of exogenous G beta subunits is sufficient to cause channel modulation.
G-protein mediated gating of inward-rectifier K+ channels.
The main objective of this review is to assess the current understanding of the G-protein modulation of GIRK channels and their physiological importance in mammals.