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Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit theExpand
Bruchpilot Promotes Active Zone Assembly, Ca2+ Channel Clustering, and Vesicle Release
Drosophila coiled-coil domain protein Bruchpilot-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity. Expand
Fluorescence microscopy with diffraction resolution barrier broken by stimulated emission.
The diffraction barrier responsible for a finite focal spot size and limited resolution in far-field fluorescence microscopy has been fundamentally broken. This is accomplished by quenching excitedExpand
Far-field optical nanoscopy
In 1873, Ernst Abbe discovered that the resolution of focusing (‘far-field’) optical microscopy is limited to d = λ/(2nsinα ) > 200 nm, with nsinα denoting the numerical aperture of the lens and λExpand
Far-Field Optical Nanoscopy
  • S. Hell
  • Chemistry, Medicine
  • Science
  • 25 May 2007
Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization. Expand
Direct observation of the nanoscale dynamics of membrane lipids in a living cell
The ability of stimulated emission depletion (STED) far-field fluorescence nanoscopy to detect single diffusing (lipid) molecules in nanosized areas in the plasma membrane of living cells is demonstrated. Expand
Fluorescence nanoscopy by ground-state depletion and single-molecule return
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of thoseExpand
Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index
The effect of refractive‐index mismatch, as encountered in the observation of biological specimens, on the image acquisition process in confocal fluorescence microscopy is investigated theoretically.Expand
STED microscopy reveals crystal colour centres with nanometric resolution.
Based on a far-field fluorescence-based optical super-resolution scheme – stimulated emission depletion microscopy – scientists resolve densely packed individual fluorescent colour centres insideExpand
Microscopy and its focal switch
  • S. Hell
  • Materials Science, Medicine
  • Nature Methods
  • 2008
The principles of these methods together with their differences in implementation and operation are discussed, and potential developments are outlined. Expand