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Determination of phage antibody affinities to antigen by a microbalance sensor system.
A mass sensitive microsensor method is presented that allows the estimation of antibody affinity directly from the phage supernatant and it is found that the apparent affinity obtained is in accordance with the affinity of the scFv fragment as determined by conventional equilibrium enzyme-linked immunosorbent assay (ELISA) and plasmon resonance methods.
Quartz crystal microbalance (QCM) as a device for the screening of phage libraries.
7 alpha-Biotinylated testosterone derivatives as tracers for a competitive chemiluminescence immunoassay of testosterone in serum.
The optimized steric structure and the long spacer arm of the biotinylated testosterone tracer make this chemiluminescence assay well-suited for measuring total testosterone concentration in serum, demonstrating the favorable specificity of the combination of a "nearly native" tracer with a position analog antibody.
Molecular cloning and sequencing of a murine pgk-1 pseudogene family.
6 alpha-biotinylated estrone: novel tracer in competitive chemiluminescence immunoassay of estrone in serum.
- P. Luppa, S. Hauck, I. Schwab, C. Birkmayer, H. Hauptmann
- Biology, ChemistryClinical chemistry
- 1 April 1995
This enzyme immunoassay with biotin as the primary label and enhanced chemiluminescence signaling detection performs well for determining total estrone in serum and is readily adaptable to assays for other steroid hormones.
Characterization of a human pancreatic secretory trypsin inhibitor mutant binding to Legionella pneumophila as determined by a quartz crystal microbalance.
Analysis of Protein Interactions Using a Quartz Crystal Microbalance Biosensor
ELISA, calorimetry, and fluorescence spectroscopy are well-established techniques for the investigation of protein–protein interactions but the quartz crystal microbalance (QCM) has been established as an alternative to SPR.
Application of Bioactive Layers for Chemical Sensors and Analysis Systems
Three different methods using bioactive layers for the immunological determination of the toxin staphylococcal enterotoxin B SEB are presented, two of which have a higher detection limit and a worse accuracy in comparision with the conventional microtiter plate ELISA for SEB.
Synthesis of 17 beta-hydroxyandrost-4-en-3-one-7 alpha-(biotinyl-6-N-hexylamide), a conjugate useful for affinity chromatography and for testosterone immunoassays.
The 7 alpha-biotinylated testosterone was used to obtain active antitestosterone antibodies from a specific antiserum by affinity chromatography and is an effective component for use in a competitive immunoassay for testosterone in human sera.