S. Harada

Publications325
h-index26
Citations2,662
Highly Influential Citations75
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Three-dimensional structure of Escherichia coli glutathione S-transferase complexed with glutathione sulfonate: catalytic roles of Cys10 and His106.
TLDR
It is proposed that glutathione S-transferases have diverged from a common origin and acquired different catalytic apparatuses in the process of evolution.
Structure of the trypanosome cyanide-insensitive alternative oxidase
  • T. Shiba, Y. Kido, +15 authors K. Kita
  • Biology, Medicine
    Proceedings of the National Academy of Sciences
  • 4 March 2013
TLDR
Crystal structures of the trypanosomal alternative oxidase in the absence and presence of ascofuranone derivatives reveal that the oxidase is a homodimer with the nonhaem diiron carboxylate active site buried within a four-helix bundle.
Unraveling the heater: new insights into the structure of the alternative oxidase.
TLDR
Recent advances in understanding of this protein's structure are described following the recent successful crystallization of the alternative oxidase from T. brucei and a mechanism for the reduction of oxygen to water is proposed based on structural insights.
Structure of the zinc endoprotease from Streptomyces caespitosus.
TLDR
X-ray structural analysis of ScNP revealed that despite a lack of sequence homology, the common topological feature of main-chain folding and a beta-turn containing methionine, which is a feature of the zinc metalloendoprotease superfamily of metzincins, is conserved in ScNP.
Simple Colorimetric Inhibition Assay of Heme Crystallization for High-Throughput Screening of Antimalarial Compounds
TLDR
A simple colorimetric assay using Tween 20 as an initiator and a microplate reader for high-throughput screening of inhibitors of β-hematin formation is developed.
A novel mode of target recognition suggested by the 2.0 A structure of holo S100B from bovine brain.
TLDR
The molecular structure of holo S100B suggests a novel mode of target recognition for the S100 family of calcium-binding proteins.
Expression, Purification, and Characterization of AknX Anthrone Oxygenase, Which Is Involved in Aklavinone Biosynthesis in Streptomyces galilaeus
TLDR
Theexpression of aknX in Escherichia coli indicates that AknX and related gene products such as DnrG and SnoaB are involved in the formation of aklanonic acid from its anthrone precursor, as suggested by their homology with TcmH and ActVA6.
Alcohols induce beta-hematin formation via the dissociation of aggregated heme and reduction in interfacial tension of the solution.
TLDR
The results showed that the ability of alcohols to induce beta-hematin formation in the order: n-butanol>n-propanol>ethanol>methanol>, suggesting that the dissociation of aggregated heme by alcohols is a major factor in beta-hematicin formation.
Kinetic characterization of methionine γ‐lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against…
Methionine γ‐lyase (MGL) (EC 4.4.1.11), which is present in certain lineages of bacteria, plants, and protozoa but missing in mammals, catalyzes the single‐step degradation of sulfur‐containing amino…
Critical importance of the de novo pyrimidine biosynthesis pathway for Trypanosoma cruzi growth in the mammalian host cell cytoplasm.
TLDR
The de novo pyrimidine pathway is important for proliferation of T. cruzi in the host cell cytoplasm and represents a promising target for chemotherapy against Chagas disease.
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