Structure of the trypanosome cyanide-insensitive alternative oxidase
- T. Shiba, Y. Kido, K. Kita
- Biology, ChemistryProceedings of the National Academy of Sciences
- 4 March 2013
Crystal structures of the trypanosomal alternative oxidase in the absence and presence of ascofuranone derivatives reveal that the oxidase is a homodimer with the nonhaem diiron carboxylate active site buried within a four-helix bundle.
Unraveling the heater: new insights into the structure of the alternative oxidase.
- A. Moore, T. Shiba, Luke Young, S. Harada, K. Kita, Kikukatsu Ito
- BiologyAnnual Review of Plant Biology
- 2 May 2013
Recent advances in understanding of this protein's structure are described following the recent successful crystallization of the alternative oxidase from T. brucei and a mechanism for the reduction of oxygen to water is proposed based on structural insights.
Three-dimensional structure of Escherichia coli glutathione S-transferase complexed with glutathione sulfonate: catalytic roles of Cys10 and His106.
- M. Nishida, S. Harada, S. Noguchi, Y. Satow, H. Inoue, K. Takahashi
- Biology, ChemistryJournal of Molecular Biology
- 7 August 1998
It is proposed that glutathione S-transferases have diverged from a common origin and acquired different catalytic apparatuses in the process of evolution.
Simple Colorimetric Inhibition Assay of Heme Crystallization for High-Throughput Screening of Antimalarial Compounds
A simple colorimetric assay using Tween 20 as an initiator and a microplate reader for high-throughput screening of inhibitors of β-hematin formation is developed.
A novel mode of target recognition suggested by the 2.0 A structure of holo S100B from bovine brain.
Structure of the zinc endoprotease from Streptomyces caespitosus.
X-ray structural analysis of ScNP revealed that despite a lack of sequence homology, the common topological feature of main-chain folding and a beta-turn containing methionine, which is a feature of the zinc metalloendoprotease superfamily of metzincins, is conserved in ScNP.
Expression, Purification, and Characterization of AknX Anthrone Oxygenase, Which Is Involved in Aklavinone Biosynthesis in Streptomyces galilaeus
- Jin Chung, I. Fujii, S. Harada, U. Sankawa, Y. Ebizuka
- BiologyJournal of Bacteriology
- 15 November 2002
Theexpression of aknX in Escherichia coli indicates that AknX and related gene products such as DnrG and SnoaB are involved in the formation of aklanonic acid from its anthrone precursor, as suggested by their homology with TcmH and ActVA6.
Alcohols induce beta-hematin formation via the dissociation of aggregated heme and reduction in interfacial tension of the solution.
Structural Insights into the Molecular Design of Flutolanil Derivatives Targeted for Fumarate Respiration of Parasite Mitochondria
- D. Inaoka, T. Shiba, S. Harada
- Biology, ChemistryInternational Journal of Molecular Sciences
- 1 July 2015
It is found that flutolanil is an excellent inhibitor for A. suum complex II but less effectively inhibits homologous porcine complex II, and structures of complex IIs binding these compounds will be helpful to accelerate structure-based drug design targeted forcomplex IIs.