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A Novel In Vivo Assay Reveals Inhibition of Ribosomal Nuclear Export in Ran-Cycle and Nucleoporin Mutants
TLDR
Nuclear export of ribosomes requires the nuclear/cytoplasmic Ran-cycle and distinct nucleoporins and overexpression of dominant-negative RanGTP and the tRNA exportin Los1p inhibits ribosomal export. Expand
The Schizosaccharomyces pombe protein Yab8p and a novel factor, Yip1p, share structural and functional similarity with the spinal muscular atrophy-associated proteins SMN and SIP1.
TLDR
The analysis of the Schizo-saccharomyces pombe protein Yab8p is described and it is shown that it is structurally and functionally related to SMN found in higher eukaryotes and interacts via its N-terminus with a novel protein termed Yip1p, which is likely to be the homologue of SIP1 in S.pombe. Expand
Importin-β facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels
TLDR
Using different nuclear transport assays, it is found that Ago as well as TNRC6 proteins shuttle between the cytoplasm and the nucleus, and it is demonstrated that TNRC 6 proteins are imported by the Importin-β pathway. Expand
Description of five mutants of the cyanobacterium Anabaena sp strain PCC 7120 affected in heterocyst differentiation and identification of the transposon-tagged genes.
TLDR
Five transposon-generated mutants that were not able to form mature heterocysts were characterised and three genes could be involved in formation of the heterocyst-specific envelope: alr2887, encoding a probable outer membrane efflux protein, alr3698, a glycosyl transferase and alr5357, the formerly described gene hglB/hetM, encode a fatty-acid synthetase. Expand
siPools: highly complex but accurately defined siRNA pools eliminate off-target effects
TLDR
It is demonstrated that novel, enzymatically generated siRNA pools—referred to as siPools—containing up to 60 accurately defined siRNAs eliminate off-target effects, achieved by the low concentration of each individual siRNA diluting sequence-specific off- target effects below detection limits. Expand
SMNrp is an essential pre‐mRNA splicing factor required for the formation of the mature spliceosome
TLDR
It is suggested that SMNrp, as a U2 snRNP‐associated protein, facilitates the recruitment of the [U4/U6·U5] tri‐snRNP to the pre‐spliceosome. Expand
Homogeneous time-resolved G protein-coupled receptor-ligand binding assay based on fluorescence cross-correlation spectroscopy.
TLDR
This work demonstrates the suitability of the homogeneous and time-resolved FCCS assay format for a robust, high-throughput determination of receptor-ligand binding affinities and kinetic rate constants for various therapeutically relevant GPCRs. Expand
Lithium chloride effectively kills the honey bee parasite Varroa destructor by a systemic mode of action
TLDR
Lithium chloride that was used to precipitate RNA and other lithium compounds is highly effective at killing Varroa mites when fed to host bees at low millimolar concentrations, providing a promising basis for the development of an effective and easy-to-apply control method for mite treatment. Expand
Simultaneous detection of intracellular target and off‐target binding of small molecule cancer drugs at nanomolar concentrations
TLDR
A combination of fluorescence correlation spectroscopy (FCS) and fluorescence cross‐correlation spectroscope (FCCS) is presented as a solution to the problem of off‐target binding and membrane permeability in vitro assays. Expand
The in vitro biological activity of the HLA-DR-binding clinical IgG4 antibody 1D09C3 is a consequence of the disruption of cell aggregates and can be abrogated by Fab arm exchange.
TLDR
The results indicate that the activity of 1D09C3 in vitro may have been a consequence of assay design rather than an ability to induce HLA-DR-dependent cell death, and this antibody undergoes Fab arm exchange in the presence of IgG4. Expand
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