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Crystal structures of human DNA polymerase beta complexed with gapped and nicked DNA: evidence for an induced fit mechanism.
Crystal structures suggest that pol beta may enhance fidelity by an induced fit mechanism in which correct base pairing between template and incoming dNTP induces alignment of catalytic groups for catalysis (via thumb closure), but incorrect base pairing will not. Expand
Structures of ternary complexes of rat DNA polymerase beta, a DNA template-primer, and ddCTP.
Two ternary complexes of rat DNA polymerase beta, a DNA template-primer, and dideoxycytidine triphosphate have been determined at 2.9 A and 3.6 A resolution, suggesting that the polymerase-DNA-ddCTP interactions are not affected by crystal packing forces. Expand
Requirement of mammalian DNA polymerase-beta in base-excision repair.
It is demonstrated that beta-polymerase functions specifically in base-excision repair in vivo, and is rescued following stable transfection with a wild-type beta- polymerase minitransgene. Expand
FEN1 Stimulation of DNA Polymerase β Mediates an Excision Step in Mammalian Long Patch Base Excision Repair*
It is demonstrated by immunodepletion experiments that 5′-dRP-N3excision in long patch BER of uracil-DNA in a human lymphoid cell extract is, indeed, dependent upon FEN1 and that human F EN1 and β-pol can cooperate in longPatch BER excision and specify the predominant excision product seen with a cell extract. Expand
Different DNA polymerases are involved in the short- and long-patch base excision repair in mammalian cells.
A novel model whereby the two BER pathways are characterized by different protein requirements, and a functional redundancy at the level of DNA polymerases provides cells with backup systems is lead to. Expand
DNA polymerase beta -mediated long patch base excision repair. Poly(ADP-ribose)polymerase-1 stimulates strand displacement DNA synthesis.
The DNA synthesis and flap excision steps in long patch BER are examined using a reconstituted system containing a 34-base pair BER substrate and five purified human enzymes: uracil-DNA glycosylase, apurinic/apyrimidinic endonuclease, DNA polymerase beta, flap end onuclease-1 (FEN-1), and PARP-1. Expand
Specificity and mechanism of error-prone replication by human immunodeficiency virus-1 reverse transcriptase.
The results suggest that the formation and/or utilization of misaligned template-primers is increased during the dissociation-reinitiation phase of the reaction, and base substitution and one-base frameshift mutational hot spots are observed. Expand
Mammalian base excision repair and DNA polymerase beta.
Crystal structure of rat DNA polymerase beta: evidence for a common polymerase mechanism.
The two invariant aspartates found in all polymerase sequences and implicated in catalytic activity have the same geometric arrangement within structurally similar but topologically distinct palms, indicating that the polymerases have maintained, or possibly re-evolved, a common nucleotidyl transfer mechanism. Expand
Mammalian base excision repair by DNA polymerases delta and epsilon.
The data show for the first time that Pol delta and/or Pol epsilon are directly involved in the long-patch BER of abasic sites and might function as back-up system for Pol beta in one-gap filling reactions. Expand