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ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage
TLDR
A large-scale proteomic analysis of proteins phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR is performed and more than 900 regulated phosphorylation sites encompassing over 700 proteins are identified.
A PGC1-\(\alpha\)-dependent Myokine that Drives Brown-fat-like Development of White Fat and Thermogenesis
TLDR
It is shown that PGC1α expression in muscle stimulates an increase in expression of Fndc5, a membrane protein that is cleaved and secreted as a new hormone, irisin, which results in improvements in obesity and glucose homeostasis and could be a protein therapeutic for human metabolic disease and other disorders that are improved with exercise.
Stress-Dependent Regulation of FOXO Transcription Factors by the SIRT1 Deacetylase
TLDR
One way in which members of the Sir2 family of proteins may increase organismal longevity is by tipping FOXO-dependent responses away from apoptosis and toward stress resistance.
Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry
TLDR
This work clarifies the preferred methodology by addressing four issues based on observed decoy hit frequencies: the major assumptions made with this database search strategy are reasonable, concatenated target-decoy database searches are preferable to separate target and decoydatabase searches, and the theoretical error associated with target-Decoy false positive (FP) rate measurements can be estimated.
Identification of a Unique TGF-β Dependent Molecular and Functional Signature in Microglia
TLDR
It is found that TGF-β was required for the in vitro development of microglia that express the microglial molecular signature characteristic of adultmicroglia and that microglian were absent in the CNS of TGF -β1–deficient mice.
Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1.
TLDR
It is shown that the Sir2 homologue, SIRT1 controls the gluconeogenic/glycolytic pathways in liver in response to fasting signals through the transcriptional coactivator PGC-1alpha, and this finding has strong implications for the basic pathways of energy homeostasis, diabetes and lifespan.
A PGC1α-dependent myokine that drives browning of white fat and thermogenesis
TLDR
It is shown in mouse that PGC1-α expression in muscle stimulates an increase in expression of FNDC5, a membrane protein that is cleaved and secreted as a newly identified hormone, irisin, which results in improvements in obesity and glucose homeostasis.
Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
TLDR
An approach for the accurate quantification and concurrent sequence identification of the individual proteins within complex mixtures based on isotope-coded affinity tags and tandem mass spectrometry is described.
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